油菜是我国重要的油料作物，需磷较多，对缺磷很敏感。局部施磷不仅能够减少磷的固定，还能促进根系发生发育，提高磷的吸收效率，显著增加产量。油菜是异源四倍体，基因组比较复杂，因此油菜响应土壤磷有效性的信号通路研究相对滞后。本研究拟以长江中下游油菜推广品种中双11号为研究对象，采用土培盆栽和琼脂平板培养分根试验，研究局部供磷对油菜根系和地上部生长以及磷等养分吸收的影响；利用转录组测序技术挖掘油菜不施磷处理、局部施磷处理和全部施磷处理的根系和地上部差异表达基因，构建油菜响应局部供磷的信号转导和基因调控网络；明确2-3个关键基因的功能，初步揭示油菜响应局部供磷的分子机理。研究结果将为通过“局部施磷促根”实现油菜“减磷增效”提供重要的理论基础。

Oilseed rape (Brassica napus L.) is one of the most important oil crops in China, which needs more phosphorus (P) and shows more sensitive to phosphate (Pi) starvation than other crops. Local Pi supply could not only reduce the P fixation by the soil, but also promote the root development, improve P uptake efficiency and significantly increase the crop yield. Brassica napus is an allotetraploid species with complex genome. The research progresses on the signal pathway of Brassica napus in response to Pi bioavailability is relatively lagging behind. In this study, the Brassica napus cultivar Zhongshuang 11, which is popularly cultivated in the middle and downstream area of the Yangtze River, is employed to study the effects of local Pi supply on the root and shoot growth and P uptake in Brassica napus by the split-root experiment (agar culture and pot culture). RNA seq. is used to dissect the differentially expressed genes in the root and shoot of Brassica napus among the treatments of no Pi supply, local Pi supply and Pi supply, and used to construct the signal transduction and gene regulation network in Brassica napus under local Pi supply. The function of 2-3 key genes and transcription factors will be clarified and the molecular mechanisms of Brassica napus in response to local Pi supply will be revealed. The results of this study will provide an important theoretical basis for “reduce Pi apply and increase yield” of oilseed rape by "promoting roots growth with local Pi supply ".