组织工程血管化是目前再生医学领域的研究热点与难点，DNA四面体纳米材料（TDNs）是新型的纳米材料，由于其尺度和结构可控、产率高、生物相容性好，成为再生医学研究的新热点，其在组织工程血管化方面应用未见报道。本课题组前期研究证实TDNs在特定尺度、特定序列和特定浓度时，可以促进内皮细胞的体外增殖、迁移和形成血管腔样结构。本项目着眼于TDNs及复合KLT多肽的TDNs（TDNs-KLT），探究其对小鼠来源血管内皮细胞增殖、迁移等生物学行为的调控作用；采用基因芯片、细胞转染等技术明确参与调控的关键分子机制和动态变化规律；通过体内外二维、三维凝胶成血管模型的构建，进一步探究TDNs及TDNs-KLT纳米材料对内皮细胞介导的新生血管形态、数量、密度和功能的影响，以期加快功能性血管系统的形成，为组织工程血管化研究及机体组织缺损的修复与再生寻找新的思路和方法。

Tissue engineering vascularization plays a critical role in the field of regenerative medicine. Owing to the merits including negligible immunogenicity, natural biocompatibility, structural stability and unsurpassed programmability, DNA tetrahedral nanomaterials (TDNs)- the novel nanomaterials, show promising potentials in regenerative medicine. Recent progress has witnessed that DNA nanomaterials have been successfully applied in the field of biomedicine and bio-imaging, however, no in-depth study of DNA tetrahedral nanomaterials and tissue engineering vascularization has been reported. Our previous studies demonstrated that interventions of TDNs with specific concentrations can effectively regulate angiogenesis mediated by endothelial cells. Therefore, this project focuses on TDNs and a novel delivery system- TDNs ligated with KLT peptides (TDNs-KLT), which is capable to activate VEGF signaling pathway in angiogenesis, aims to explore how TDNs and TDNs-KLT modulate biological behaviors such as proliferation and migration of mouse-derived vascular endothelial cells. Meanwhile, DNA microarray and cell transfection techniques are used to clarify the underlying molecular mechanisms. Finally, two-dimensional and three-dimensional vascular-forming models are established to explore the effects of TDNs and TDNs-KLT on vascular quantity, density and functions in the endothelial cell-mediated angiogenesis. The results will contribute to accelerate the formation of functional vascular system and pave a novel way for the study of vascularization in tissue engineering and regeneration.