随着纳米材料的广泛应用，其对生态环境的负效应日益受到关注。传统毒性评价方法集中在作用末期评价毒性，纳米颗粒作用浓度高，难以评估纳米颗粒物在低剂量长期赋存条件下对生物的作用，尤其忽视了可能存在的hormesis效应。本研究将研究不同水介质中C60纳米颗粒物对Daphnia的hormesis效应及分子机制。研究首先通过Daphnia magna的体重、体长、繁殖和寿命识别hormesis特征，建立剂量/效应模型；基于GC-TOF和LC-Q-TOF的检测技术平台建立代谢组学研究方法体系，通过外源性及内源性代谢产物的检测，并结合关键酶活性探究C60纳米颗粒物对Daphnia magna产生hormesis效应的分子作用机制；建立多参数统计分析模型，寻找识别hormesis效应的特征代谢标志物。研究对纳米颗粒物在低剂量、长期暴露条件下hormesis分子机制识别及对水生生物的风险评估具有重要意义.

Ecological environmental implications of manufactured nanomaterials have received.increasing attentions with their wide applications in various fields. Current.evaluation approaches measure mortality and reproductive or growth effects at the.endpoint. Since concentration of nanomaterials is higher than environmentally.relevant concentration, it is difficult to evaluate the long-term biological effect of nanomaterials at low concentration, and their inability to provide information on modes of toxicity, and also the ignorance of potential hormesis. Under such a circumstance, this research project will work on the hormesis of aqueous C60 nanoparticles on Daphnia Magna and its molecular mechanisms. The 3 main modules include: first for the characterization of the hormesis by the weight, length, reproduction, life span of Daphnia magna;second for the establishment of metabolomics methodology based on GC-TOF and LC-Q-TOF to detect exogenous metabolites and endogeneous metabolites, and combination with some key enzymes to identify the molecular mechanism of hormesis; third for the development of multi-parameter statistic analysis model to identify the biomarker of hormesis. This project is of significance for distinguishing hormetic mode of action, discovering the biomarker, and risk assessments of nanomaterials at low concentrations.