聚合物/纳米粒子/二氧化硅三明治结构的新型手性固定相的制备及分离手性药物对映体的研究

为了改善传统硅胶基质HPLC填料的手性拆分能力和分离选择性，克服硅胶机械强度低，不耐酸碱，比表面积和样品上载量有限的缺陷，本课题将纳米粒子和手性聚合物引入传统硅胶填料，制备一种新颖的三明治结构的手性HPLC固定相。即以微米硅胶为核，通过分子层层自组装法将不同粒径的金、银、硅纳米粒子分别负载于硅胶表面作中间夹层；为了防止纳米粒子流失，保证填料稳定性，然后通过聚合反应在纳米粒子表面形成聚合物网状结构作外壳；最后植入环糊精衍生物，得到手性聚合物/纳米粒子/二氧化硅三明治结构的手性固定相。这种结构的色谱填料结合传统填料和纳米材料的优点，对对映体的拆分具有协同效应。将其用于生物碱等重要的手性药物对映体的拆分，并系统的研究该固定相的拆分机理以及纳米材料在手性对映体拆分中的作用和行为。

In order to achieve higer enantioseparation efficency and selectivity, and overcome unavoidable weakness of tradition silica-based stationary phase from low mechanical strength,not resistant to acid and alkali, specific surface area, and sample capacity, the surface of silica sphere will be modified using nanoparticles and chiral polymer. A novel HPLC chiral stationry phase with a sandwich stucture will be prepared. Firstly, μm-sized silica spheres are used as a core.Secondly, nm-sized particles including AuNP，AgNP and SiNP with different particle size, are packed on the surface of silica spheres by a layer-by-layer self-assembly approach. Thirdly,in order to provent release of nanoparticles from the column with different moblie phase and keep clumn stability, the net structure of polymer is formed on the surface of nanoparticle by polymerizatiom reaction. Finally, derivatized cyclodextrin is embedded in the polymer chains.The polymer/nanoparticles/silica chiral stationary phase with a sandwich structure will be obtained, which is a powerful combination of traditional packing and nano materials.Moreover,nanoparticles have a synergistic effect with chiral polymer for enantioseparation. Additionally, it will be used for enantiomeric separation of important chiral drugs such as alkaloids.The mechanisms of chiral recognition and acts of nanomaterials in enantiomeric separation will be also studied.