一个新的根结线虫效应蛋白Mg-mo237抑制植物防卫反应的机理研究

植物受病原物侵入后，可触发病原相关的分子模式触发免疫（PTI）和效应蛋白触发免疫（ETI）两种防卫反应以阻止病原物入侵。病原物则可分泌抑制植物防卫反应的效应蛋白来成功寄生植物。根结线虫是最重要的植物病原线虫之一，其食道腺分泌的效应蛋白可抑制植物防卫反应。本项目以本课题组从拟禾本科根结线虫中获得的、并已通过实验证明与寄主植物的胼胝质合成酶CSL8相互作用且被干扰后会降低线虫寄生能力的一个新的效应蛋白Mg-MO237为研究对象，利用发育表达模式和免疫组化方法研究Mg-MO237在线虫中的表达及分泌特性、并通过RNAi、过表达、BIFC、Co-IP和qPCR等方法研究Mg-MO237和CSL8的相互作用，对根结线虫寄生的影响及对植物胼胝质、PTI和ETI等的影响。本项目有望明确Mg-MO237效应蛋白与植物胼胝质的关系和对寄主防卫反应的影响，揭示根结线虫MO237蛋白抑制植物防卫反应的作用机理。

The defence system of plants including PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI) would be induced when plants are attacked by plant pathogens. And plant pathogens could secrete effectors to suppress the defence of plants to enable successful infection. Root-knot nematodes (Meloidogyne spp.) are one of the most damaging plant-parasitic nematodes. There are direct evidences that secretory proteins originating from esophageal glands of Meloidogyne can suppress the defence of plants. In this project, a novel effector protein named Mg-MO237 from M. graminicola had been proved to interact with callose synthase 8-like (CSL8) of host plant using yeast two-hybrid system and play a role in parasitism of nematodes through RNAi experiments by us. The main contents of this project would be: to analyze spatial and developmental expression and secreted characteristics of Mg-MO237 using qPCR and immunofluorescence staining; to study the interaction of Mg-MO237 and CSL8, then illustrate how Mg-MO237 affects callose, PTI and ETI using RNAi, overexpression, BIFC, Co-IP and qPCR, etc. The present project is likely to illustrate relationship of Mg-MO237 and callose, then how to affect plant defence. Finally, mechanisms of suppressing host defence by MO237 proteins from Meloidogyne would be known.