长链非编码RNA(lncRNA)是ncRNA家族的重要成员，当前研究所发现的lncRNA复杂而重要的生物学功能为研究者展现了一个新的调控领域。相对于lncRNA的大量发现和鉴定，其功能和机制仍有待进一步阐明，尤其是造血系统中lncRNA的研究仍处于起步阶段。本课题从定位于造血单核系分化重要转录因子CEBPA基因座旁侧的lncRNA：linc-CEBPG入手，在发现其与RNA结合蛋白KSRP结合并负调控CEBPA表达，进而抑制单核系分化的基础上，进一步明确linc-CEBPG和KSRP的生物学功能；确定两者相互作用参与调控单核系分化的机制；研究两者与CEBPA的内在联系及调控CEBPA表达的详细分子机制；并在单核系白血病病例中研究linc-CEBPG和KSRP的作用机理和诊疗价值。以上结果将进一步完善lncRNA的调控模式和作用机理，并为ncRNA:蛋白双色调控理论提供新的证据。

Long non-coding RNA (lncRNA) is the essential component of non-coding RNA family, which has been implicated in various cellular processes. However, the underlining mechanisms, especially their involvement in hematopoiesis and leukemogenesis are largely unknown. In this work, we select potential lncRNAs to study based on their genomic location and identify a candidate flanking the master monocytic transcription factor CEBPA, named linc-CEBPG. We have found that linc-CEBPG down-regulates the expression of CEBPA through binding with RNA binding protein KSRP and inhibits monocytic differentiation. We will further assess their regulatory roles in monocytic differentiation and confirm the interaction between linc-CEBPG and KSRP, and evaluate their cooperativity in regulating monocytic differentiation. Given the cis-acting of lncRNA on its adjacent genomic locus, we aim to illustrate the effect of linc-CEBPG/KSRP interaction on CEBPA expression, and clarify the molecular mechanism of the regulation. In addition, we will further study the involvement of linc-CEBPG and KSRP in leukemogenesis. These results will provide new insights into lncRNA study and strengthen our understanding of ncRNA:protein interacting model.