目前研究认为，牙骨质的发育分化调控在牙周组织再生中起重要作用。在前期研究中，本课题组发现Wnt信号通路参与牙骨质中的发育调控，Osx通过DKK1调控Wnt信号通路在成牙骨质细胞分化中起重要作用；同时发现炎性状态下，miR-155-3p也通过靶向Kctd1，调控Wnt信号通路参与成牙骨质细胞的分化；此外通过生物信息学预测 lncRNA-Gm10435能够与miR-155-3p结合，进一步预实验研究也证实lncRNA-Gm10435与TNF-α炎性诱导时间呈负相关。基于上述研究背景，我们提出炎性环境下lncRNA-Gm10435相关的ceRNA网络参与miR-155-3p/经典Wnt信号通路调控成牙骨质细胞分化的机制研究，为此本课题将深入研究体内外炎性状态下这一调控网络对牙骨质形成和矿化的影响，为牙骨质的发育分化的精准调控及牙周组织的再生提供新的切入点和方向。

Currently, many studies showed that the development and differentiation of cementum had critical role in the regeneration of periodontal tissues. Our recent previous study demonstrated the expression of Wnt in cementum as well as the important role of Osx on cementoblast differentiation by regulating Wnt signaling pathway via DKK1. At the same time, we also found that miR-155-3p could participate in cementoblast differentiation by targeting Kctd1 via regulating Wnt signaling pathway. Besides, bioinformatic prediction found that lncRNA-Gm10435 could competitively bind to miR-155-3p; further pre-test by us also discovered the negative correlation of lncRNA-Gm10435 with TNF-α induced inflammation on time levels. Based on above facts, we put forward the hypothesis that lncRNA-Gm10435-related ceRNA network might be involved in cementoblast differentiation under inflammatory conditions by regulating miR-155-3p/canonical Wnt signaling pathway. Hence, on one hand, we will investigate the effect of lncRNA-Gm10435 on cementoblast differentiation by gain/lose-of-function experiments. On the other hand, we will lucubrate the ceRNA regulatory network under inflammatory conditions in vivo and in vitro. The effects of lncRNA-Gm10435/miR-155-3p/Wnt signaling pathway on the formation and mineralization of cementum may guide a great direction of cementum regeneration.