课题组前期研究显示胸腺基质淋巴生成素（TSLP）参与了大疱性类天疱疮（BP）的发病过程，且Tfh与BP病情相关。但TSLP是否通过Tfh在BP发病中发挥作用？故提出假说“在BP的发病过程中，TSLP能通过活化树突状细胞，激活Tfh细胞，引起Th2免疫反应，进而促使B细胞增殖、抗体分泌升高，产生正反馈循环，从而导致疾病进展”。本研究拟从分子和细胞水平探究皮肤组织内TSLP的来源及效应细胞类型；在患者水平证实TSLP在BP患者组织含量升高，且与病情相关。抗TSLP单抗治疗后，可大大缓解疾病严重程度。该研究从分子-细胞-患者三个层次开展系统研究，实现抗TSLP治疗BP的转化研究路径。旨在揭示TSLP、Tfh在BP发病中的作用机制，为寻找早期监测靶点及治疗靶点提供理论依据。

Previous studies in our group have shown that thymic stromal lymphopoietin (TSLP) is involved in the pathogenesis of bullous pemphigoid (BP), and follicular helper T cells （Tfh） is related to the severity of BP. By reviewing the literature and our previous studies, we hypothesized that "in the pathogenesis of BP, TSLP can activate dendritic cells (DC) and Tfh cells to induce Th2 immune response, and then promote the proliferation of B cells, increase the secretion of antibodies, and produce positive feedback cycle, thus leading to disease progression". The purposes of this study are (1) to explore the source and effector cell of TSLP in skin tissues at the molecular and cellular levels; (2) to confirm that the levels of TSLP in the serum and bullae may increase in BP patients. After treatment with anti-TSLP monoclonal antibody, the severity of the disease can be significantly alleviated. In this study, we will carry out a systematic study on the three levels: molecular-cell-human and perform the typical translational medicine research path. The final purpose of this study is to reveal the mechanism of TSLP and Tfh in the pathogenesis of BP, and to provide theoretical basis for finding early monitoring targets and therapeutic targets.