雌性体内精子储存是动物提高自身繁殖成功率的一种特殊生殖策略，对物种的交配机制演化及行为进化具有重要意义。短蛸（Amphioctopus fangsiao）作为我国北方重要的经济种类，其生命周期短，精子在纳精囊内储存长达7个月，为研究章鱼类精子储存机制的理想物种。本项目拟利用ELISA、Western Blot等免疫标记技术，深入系统的研究雄激素在短蛸精子储存中的作用机制。明确雄激素对精子储存的调控作用；揭示纳精囊上皮细胞凋亡、自噬与精子储存的关系；通过分析雄激素水平对纳精囊精子储存、细胞凋亡与自噬的影响，阐明雄激素是否通过影响纳精囊细胞凋亡、自噬途径调节精子储存。研究结果有助于了解头足类的交配机制演化及行为进化趋势，并为短蛸的人工繁育、资源增殖与保护提供科学依据。

Sperm storage in female species is a special reproductive strategy to improve the reproductive success, and has important implications for mating system and behaviors evolution. Amphioctopus fangsiao, as an important economic species in north China, has a short life cycle and sperm can be stored in spermatheca for up to 7 months. It is an ideal species to study the sperm storage mechanism of octopus. In this project, the mechanism of androgens in sperm storage of A. fangsiao will be studied using ELISA,Western Blot and other immunomarker techniques. This project seeks to: clarify the regulating effect of androgens on sperm storage; reveal the relationship between spermatheca epithelial cell apoptosis, autophagy and sperm storage; ascertain whether androgens regulate sperm storage through the apoptotic, autophagy pathway by analyzing the effect of androgens level on sperm storage and cell apoptosis, autophagy in spermatheca. The proposed project will be helpful to understand the evolution of mating system and behaviors of cephalopods, and provide scientific basis for artificial breeding, resource proliferation and protection of A. fangsiao.