Msc-1 基因调控辣椒 GMS 育性的分子机理研究

雄性不育在辣椒杂种优势利用中有重要的应用价值。由于辣椒 GMS 利用时父本来源不受限制，有利于优良杂交品种的选育，且雄性不育较稳定，因此应用前景较为广阔。本项目在克隆到辣椒 GMS 育性控制基因 Msc-1 的基础上，进一步通过时空表达分析、亚细胞定位、VIGS 和遗传转化（基因敲除技术和纳米磁转化法等）辣椒等技术，明确 Msc-1 的功能；同时，结合转录组和蛋白组学分析探索 Msc-1 调控的基因网络，筛选与 Msc-1 有关的上下游候选基因和蛋白，通过蛋白互作技术、相关基因的表达分析和 VIGS 基因沉默技术等验证 Msc-1 与这些基因的互作关系。从而解析 Msc-1 调控辣椒 GMS 育性的分子机理。为辣椒育性调控和 GMS 在辣椒杂种优势中的利用提供理论依据。

Male sterility has an important application value in the utilization of pepper heterosis. Due to the widely sources of male parent in breeding which is beneficial to cultivate improved varieties, and the fertility of GMS is more stable, the application of GMS is much promising. Based on fine mapping and cloned the fertility gene (Msc-1) in GMS, we through the spatio-temporal expression, subcellular localization, VIGS and genetic transformation (Gene knockout technique and Pollen magnetofection) of pepper to definite the function of Msc-1. In the meantime we explore the Msc-1 gene regulatory network and screen upstream and downstream genes associated with Msc-1 by RNA-seq and Proteomics. And then verified the relationships of these genes with Msc-1 through protein-protein interactions, qRT-PCR and VIGS in order to explore the molecular mechanism of Msc-1 in regulating the GMS fertility in pepper. Taken together it can provide theoretical foundation for regulating the fertility and application value in the utilization of GMS in pepper.