口腔黏膜白斑是口腔癌最重要的来源之一，白斑癌变的早期诊断和治疗的分子标志物亟待研究。Podoplanin (PDPN)是目前研究最具影响的白斑癌变标志物；申请人在前期工作中发现PDPN在口腔红斑和扁平苔藓癌变组织中呈异常高表达，提示PDPN可能是癌变标志物，但其调控机制尚不明了。长链非编码RNA (lncRNA)是癌发生重要的调控分子，但与癌变相关lncRNA及其与PDPN相关的研究尚属空白。本项目利用癌变患者在随访中所采集的标本，构建白斑癌变进程中相关lncRNA的表达谱；鉴定能调控PDPNover细胞功能的目标lncRNA，验证目标lncRNA对PDPNover白斑细胞克隆形成、细胞增殖、周期、分化、凋亡等细胞功能的影响。通过本项目的研究，可明确白斑癌变相关lncRNA表达谱及其能调控PDPN过表达的lncRNA，并为PDPNover靶向治疗提供lncRNA新靶点。

Oral leukoplakia (OLK) is one of the most important source of oral cancer. Molecular markers of early diagnosis and treatment for malignant transformation of OLK are urgently needed. Currently, podoplanin (PDPN) is the most influential markers. Our previous work showed that PDPN abnormally high expression in progressive oral erythroplakia and lichen planus, further supporting PDPN may be the marker, but regulatory mechanism is unclear. Long non-coding RNA (lncRNA) is the important regulatory molecules of cancer development. However the research on with transformation of OLK related lncRNA and PDPN still is blank. Based on previous work, the aim of the project is to utilize the specimens collected from patients with OLK transformation in the follow-up, identity the lncRNA expression profile of OLK transformation and the target lncRNA of regulation PDPNover cell function, verify that the target lncRNA influences the PDPNover cell colony formation, cell proliferation, cycle, differentiation, apoptosis. Through the project, we canclarify OLK transformation related lncRNA expression and lncRNA of regulation PDPN over expression, and provide lncRNA new target for PDPNover targeted therapy.