新型包载bFGF的两性离子多孔水凝胶敷料的制备以及对糖尿病型慢性难愈合创面修复的研究

糖尿病慢性创面修复是临床亟待解决的难题之一。同时长期开放的慢性创面也极易感染各种病原体入侵进一步加重病情。目前，临床已成功将外源性生长因子bFGF应用于糖尿病创面修复治疗，并取得良好疗效。然而作为一种多肽类蛋白质药物，bFGF易被蛋白酶水解，导致其生物利用度的下降和医疗成本的提高。因此开创新型的创面敷料并可控释放bFGF具有重要的科学意义和临床价值。本项目采用具有优秀抗蛋白质非特异性吸附的SBMA两性离子材料为水凝胶敷料基质，通过传统的冷冻干燥法获得一系列不同孔径的三维水凝胶支架结构，进一步包载bFGF使bFGF与外界酶解环境相对隔离，提高bFGF稳定性，增强bFGF生物学效应；最后探索研究此包载bFGF的超亲水性两性离子SBMA水凝胶对糖尿病小鼠慢性难愈合创面的治疗修复效果。本研究将为糖尿病慢性创面提供新型的敷料同时提高bFGF生物药物的治疗水平。

Diabetic chronic wounds has become an urgent problem in clinical. And the lasting open wound further rise higher risk of inflammations of chronic wounds with more severe delay of wound regeneration. Nowadays, exogenous of growth factor bFGF has been successfully applied to treat diabetic skin wound and achieved good curative effect. However, bFGF as a kind of basic polypeptide protein drug, could be easily hydrolysis by protease limiting its stability and bio-activity in clinical applications. Thus, novel bio-materials which could control release bFGF is needed for clinical applications. Here, in this project, we incubate bFGF using zwitterionic SBMA hydrogel in order to improve bFGF’s stability and bio-activity towards chronic wound. And different 3D porous structure of hydrogel were fabricated through freeze dried upon varied cross-linking percentages to control release bFGF. Furthermore, diabetic db/db chronic wound mouse model is further established to fully study the healing ability of this composite bFGF-SBMA hydrogel. The results of this research will provide a new chronic wound dressing systems and enhance the level of bFGF drug treatment for wound.