根内定殖的内生解淀粉芽孢杆菌CC09与小麦的互作机制及对Ggt侵染的生防机理

解淀粉芽孢杆菌是重要的生防菌，具有促进植物生长和防病双重作用。但对该菌在植物体内的生物学作用、与植物的互作关系以及防病机理尚不清楚。本项目以能够在小麦根内大量定殖且产生抗菌脂肽Iturin A的内生解淀粉芽孢杆菌CC09为材料，采用组织学、生物化学、分子生物学、转录组学和生物信息学等方法，通过研究：(1)CC09在小麦根部不同组织和茎、叶器官中的分布、扩展过程、种群消长动态及抗菌脂肽Iturin A的合成能力；(2)小麦对CC09根内定殖的组织学、生物化学和分子生物学响应；(3)CC09根内定殖不同阶段其自身与小麦根组织的转录组变化；(4)CC09根内定殖对小麦抵御禾顶囊壳小麦变种(Ggt)侵染的影响，阐明CC09在小麦根内定殖的生物学特性及小麦对该菌定殖过程的应答机制，揭示CC09与小麦互作的分子基础及其对Ggt侵染的生防机制，并为研究其它内生菌与植物互作的分子机制提供新思路和新方法。

Bacillus amyloliquefaciens is an important biocontrol bacterium which performs both the roles of plant growth promotion and biocontrol. However, the biological effect of the bacterium, the relationship between the bacterium and its host plants, and the biocontrol mechanisms remain unclear. In this project, by using CC09, an endophytic B. amyloliquefaciens which shows interior colonization of wheat root and production of antifungal lipopeptide Iturin A, as the experiment material, and utiliazing histological, biochemical, molecular, transcriptomic and bioinformatic analyse, we study: (1) the distribution, spread, population dynamics, and the biosynthesis capacity of antifungal lipopetide Iturin A of the endophytic strain CC09 in root tissues,stem and leaves; (2) the histological,biochemical and molecular biological responses of wheat to the root colonization of strain CC09; (3) the transcriptomics of strain CC09 and wheat roots at various colonization stages; (4)the effect of strain CC09 colonized in roots on Ggt infection. Based on these studies, we disclose the biological traits of endophytic B. amyloliquefaciens CC09 inside of the wheat root tissues and the mechanism of how wheat responses to the colonization of the strain CC09, and reveal the molecular basis of the interaction between the strain CC09 and wheat as well as the biocontrol mechanism against Ggt infection. Moreover, the obtained results can also provide new ideas and methods for studying the the molecular mechanism of the interaction between other endophytic microbes and plants.