摘要：肉质风味性状是鸡重要的经济性状，目前对于鸡肉质风味性状的研究多集中在某个或某些基因的研究，深入揭示影响肉质风味性状基因的调控，探明优质鸡肉的遗传机理，对开发利用玫瑰冠鸡这一宝贵的遗传资源具有重要意义。本研究对玫瑰冠鸡和艾维茵鸡的肉质风味性状进行比较分析，拟利用RNA-seq和small RNA测序技术检测肌肉组织中差异表达基因和miRNA，对筛选到的差异基因及miRNA在细胞和个体水平验证其调控作用，通过miRNA-mRNA联合分析构建肉质风味性状调控通路，在此基础上对筛选的新基因设计CRISPR/Cas9敲除载体，体外检测候选基因在肉质风味形成中的功能。本研究旨在探讨肉质代谢通路中相关基因的调节机制，明确参与调控的重要基因和miRNA，对进一步了解鸡肉质风味性状的分子调控机制具有重要的促进意义。

Abstract: The meat flavor traits is one of the most important economic traits of chicken,researching on chicken’s meat flavor traits is focused on one or more genes so far,thoroughly revealing the regulation of genes influencing meat flavor traits, provening genetic mechanism of quality chickens, has important significance to the development and utilization of Rose Cockscomb Chicken —this valuable genetic resources.In this study, through comparative analysis of the differences between Rose Cockscomb Chicken and Avian Broiler’s meat flavor traits, differentially expressed genes in muscle tissue and miRNA is detected by RNA - seq and small RNA sequencing technology, differentially expressed involving-genes and miRNA’s regulatory is verified in cellular and individual level, and meat flavor traits regulatory pathway is constructed through jointing analysis of miRNA-mRNA, on this basis, the involed new genetically CRISPR / Cas9 knockout vector is designed and the candidate gene’s function in meat flavor traits emerging is detected.The purpose of this study is to explore the regulating mechanism of related genes in the metabolic pathway,clear key genes and miRNA involved in it,which has important significance in promoting further understand of molecular mechanism in chickens’ meat flavor traits.