植物乳杆菌素（plantaricin）是一类具有较宽抗菌谱的细菌素。针对目前国内外对plantaricin局限于分离鉴定、抗菌谱以及发酵优化等，而对其合成的代谢调控和启动转录的多肽诱导的分子机制研究少的问题。本研究在分离获得一株抗菌活性高的植物乳杆菌163和完成基因组测序的基础上，旨在利用基因组学、蛋白质组学和代谢组学等组学技术，分析不同培养条件下plantaricin合成代谢基因表达的差异,利用基因敲除、沉默、编辑和过表达技术，调节代谢路径中关键基因的表达量，阐明plantaricin的合成代谢调节机制。并通过对plantaricin诱导启动转录诱导物的改造，研究多肽诱导物的结构、疏水性和电荷对诱导转录效率的影响，确立plantaricin诱导转录的多肽分子模型。研究结果将为设计具有更高诱导效率的自诱导肽，优化代谢路径，提高plantaricin的产量和工业化生产提供理论依据。

Plantaricin is a class of bacteriocin with a broad antimicrobial spectrum. In view of the fact that the study of plantaricin is limited to the isolation, identification, antibacterial spectrum and the fermentation optimization. The molecular mechanism of plantaricin synthesis, metabolic regulation and induced transcription initiation is uncertain. In the previous study, Lactobacillus plantarum 163, which has high antibacterial activity, was isolated and the whole genome were sequence and analyzed. The aim at this study was to analyze the different expression levels of plantaricin synthesis gene under different culture conditions by genomics, proteomics and metabolomics. The expression of key genes in metabolic pathways was changed by gene knockout, silencing, editing and overexpression techniques, and the mechanism of metabolic regulation of plantaricin was elucidated. The molecular model of plantaricin-induced transcription was established by altering the induced peptides, studying the effect of structural model, hydrophobicity and charge on induced transcription efficiency. The results will provide a theoretical basis for improving the fermentation efficiency of plantaricin and industrial production by designing self-inducing peptides with higher induction efficiency, optimizing metabolic pathways.