fengycin是由芽孢杆菌非核糖体合成的抗菌脂肽，具有高效的抑制丝状真菌的作用，在食品防腐和植物病原真菌的防治等方面具有潜在的应用前景。国内外对枯草杆菌fengycin的发酵生产、合成酶及合成酶基因已有较多研究，但尚未阐明fengycin合成的分子调控机理。因此，对于分子水平改良fengycin生产菌株，尚缺乏信号分子调控fengycin合成的科学依据。针对这一科学问题，本研究利用fengycin生产菌株B. Subtilis ATCC9943，通过敲除和过表达枯草杆菌中信号调控蛋白的基因，探索信号调控蛋白及σ因子对fengycin合成的影响，结合荧光定量PCR和蛋白质组学的方法，推测信号调控蛋白对fengycin合成基因的转录和翻译的影响，分析与合成代谢过程的关系，揭示信号蛋白对fengycin合成的分子调控机理，为从分子水平上改造枯草芽孢杆菌提高fengycin的产量提供科学依据。

Fengycin is an antifungal lipopeptide produced by Bacillus sp. with non-ribosomal peptide synthetases. It inhibits strongly filamentous fungi and has the important applications potential in the prevention of food preservation and plant pathogenic fungi, Recently, there are a lot of studies on antimicrobial lipopeptides produced by Bacillus subtilis such as the fermentation on surfactin , fengycin, and its synthetic enzymes and synthetic enzyme genes in worldwide. There are few reports on the mechanism that signal proteins regulate on fengycin synthesis at molecular level. However, the mechanism of molecular regulation for the fengycin synthesis is unknown upto now. In order to clarify the mechanism of molecular regulation for the fengycin synthesis, The effect of knockout and overexpression of the regulatory signal protein genes, ComA, PhrC, AbrB, Spo0A, DegU and sigma factor in B. subtilis on fengycin synthesis are studied and the relationship between signal regulatory protein and transcription, expression of fengycin synthases genes or synthsis metabolism process is investigated.Our results provide a scientific basis for improving the production of fengycin at molecular level in B. subtilis.