巴西橡胶树(Hevea brasiliensis Mull.Arg.)是天然橡胶的主要来源。产（排）胶是橡胶树的创伤反应，受茉莉酸（JA）信号调控, 但目前对巴西橡胶树JA信号的机制知之甚少。我们从巴西橡胶树中克隆了10个JAZs和3个MYCs转录因子并验证它们之间互作。通过Y1H初步鉴定MYCs对某些顺式元件，如G-box具有偏好。我们利用自主开发的Hevea cis-elements scan生物信息软件，在橡胶树全基因组水平上筛选到40个启动子上携带G-box顺式元件的产（排）胶相关基因，它们很可能是MYCs调控的靶基因。本研究拟采用Y1H、EMSA、ChIP、报告基因瞬时表达以及转基因等技术，深入验证橡胶树MYCs 转录因子对产（排）胶的调控功能。目前已经找到了2个MYCs调控的靶基因，分别与产、排胶相关。我们的研究将为揭示JA信号调控巴西橡胶树产（排）胶的分子机理揭开冰山一角。

Rubber tree (Hevea brasiliensis) is the most important source of natural rubber. Latex biosynthesis and drainage are wounding responses controlled by JA signaling, but JA signaling mechanism in rubber tree is so far less known. We previously cloned 10 JAZs and 3 MYC TFs in rubber tree, respectively. The interactions among JAZs and MYCs are confirmed. We also found that MYCs have recognition bias to certain cis-elements, such as G-box elements. Using our cis-element scanning software, about 40 Hevea genes carrying at least one G-box in the promotors are predicted genomewide, which maybe candidate genes both regulated by MYCs and involved in rubber biosynthesis or drainage. EMSA, ChIP, in planta transformation and transgenic technology are going to be performed to identify MYCs' targets and functions, so as to reveal JA signaling mechanism in rubber tree.