水稻黄矮病毒M蛋白调控黑尾叶蝉多胺合成途径的分子机制

持久增殖型病毒需要通过侵染介体昆虫才能被有效传播，研究持久增殖型病毒与介体昆虫的互作机理是目前病毒学领域的热点。本实验室前期对水稻黄矮病毒RYSV的研究发现RYSV侵染后黑尾叶蝉体内多胺的含量显著提高。随后对黑尾叶蝉cDNA文库进行筛选发现RYSV编码的基质蛋白M能够与黑尾叶蝉多胺合成途径中的关键酶-鸟氨酸脱羧酶抗酶OAZ互作，由此推断RYSV很可能通过M蛋白调控黑尾叶蝉多胺合成途径，从而促进介体传毒。为进一步研究这一新的病毒与介体昆虫互作模式，本实验拟在前期获得RYSV M蛋白与介体因子OAZ互作的基础上，利用介体细胞培养、免疫荧光标记、RNAi等技术体系，在细胞和虫体水平，深入探索多胺在RYSV侵染黑尾叶蝉过程中的作用，解析RYSV调控黑尾叶蝉多胺合成途径的机制和生物学意义。以期深入解析植物核型弹状病毒通过介体昆虫扩散的机理，为探索病害的流行规律和制定有效的防控策略提供理论支撑。

It is necessary for the virus to depend on vector factors to complete infection route. And thus the studying interaction of persistent multiplication virus and its insect vector has become a recent hotspot in virology field. Our previous results indicated that the accumulation of polyamines was obviously increased during RYSV infection. By screening the leafhopper cDNA library, we found RYSV encoded matrix protein M interacted with leafhopper Ornithine decarboxylase antizyme (OAZ) which is an important enzyme in the biosynthesis of polyamines. Those results indicated that RYSV may regulate the biosynthesis of polyamines in leafhopper. To further investigate this new virus-insect interaction model, in this proposal we plan to adopt various technology systems such as vector cell culture, immunofluorescence labeling and RNAi, etc., so as to further our understanding of the multi-role of insect factors in multilaterally participating in and mediating RYSV diffusion. This project is aimed to provide a new point of view for the rice rhabdovirus study and to solid the theoretical support for exploring the epidemic law of disease and developing effective prevention and control strategies.