麝香霉属（Muscodor）真菌能够产生具有广泛抑菌活性的挥发性有机化合物（VOCs），具有极大的应用价值。然而不同麝香霉菌株产生的VOCs差异较大且易受环境影响，并且VOCs对菌株自身生长具有一定抑制作用。因此，从分子水平阐明麝香霉VOCs合成的机制以及VOCs抑制菌株自身生长的机理将为高产VOCs工程菌株的构建和改造提供理论基础。然而目前关于此方面的研究尚属空白。本研究拟以不同麝香霉菌株为研究对象进行比较基因组分析，阐明麝香霉的基因组特征，鉴定VOCs的生物合成途径，揭示不同菌株产生差异性VOCs的分子机制；并且通过转录组分析研究VOCs合成相关基因的表达以及VOCs对菌株自身产生的影响；同时，结合突变体分析鉴定VOCs抑制菌株生长相关基因并获得抗VOCs抑制的高产菌株。本项目不仅能阐明麝香霉VOCs合成及抑制自身生长的分子机制，又可以获得高产菌株，将为麝香霉的应用提供理论和菌株基础。

The most striking feature of the endophytic fungi Muscodor is their abilities to produce volatile organic compounds (VOCs). The VOCs have wide antibacterial chart and bacteriostatic activities, which are very valuable in application. However, the compositions of VOCs produced by different Muscodor spp. are diverse and easily influenced by environmental conditions. Besides, VOCs also have an inhibitive effect on the growth of Muscodor. Hence, the understanding of molecular mechanisms of the regulation of VOCs biosynthesis and growth inhibition will provide the theory basis for the construction of high yield strains. Unfortunately, the related studying is blank at present. In this project, we intend to resequence the genomes of different Muscodor stains and illuminate the VOCs biosynthetic mechanisms by comparative genome analysis. Furthermore, we will analyze the expressions of VOCs biosynthetic genes and the response of Muscodor stain under VOCs stress by transcriptome analysis. Meanwhile, by analysis on many mutants, we will clarify the mechanisms of the growth inhibition by VOCs and get the VOCs insensitive mutant strain. It is the first time to study the mechanisms for VOCs biosynthesis and growth inhibition by VOCs, and to construct the VOCs insensitive strain, which will pave the way for the application of the Muscodor.