研究证明哺乳动物体内广泛存在由大麻素受体、内源性大麻素和合成与降解内源性大麻素的酶组成的内源性大麻素系统，并在多种疾病的发生、发展中起着重要作用。本项目在前期研究成果的基础上，通过建立小鼠皮肤切创动物模型并结合原代纤维细胞培养，应用免疫荧光染色及分子生物学等技术，研究皮肤损伤后不同时间段2型大麻素受体（CB2R）的分布、蛋白和基因动态表达以及纤维细胞转化为肌成纤维细胞后CB2R的表达；应用CB2R的选择性激动剂（JWH-133）及拮抗剂（AM-630），进一步探讨皮肤损伤愈合过程中CB2R对纤维细胞（fibrocyte）转化为肌成纤维细胞、调节肌成纤维细胞分泌基质金属蛋白酶MMP-2和胶原纤维等细胞生物学功能、细胞凋亡以及相关信号通路的影响，揭示内源性大麻素系统中的CB2R在皮肤损伤愈合过程中的调控作用的分子机制,为相关药物研发奠定基础。

It is generally acknowledged that endogenous cannabinoid system is ubiquitous in mammals, which consists of cannabinoid receptors, endocannabinoids and enzymes that are reponsible for synthesizing and degrading cannabinoids. Moreover, much evidence has demonstrated that the endocannabinoid system is involved in a wide spectrum of physiological and pathological events. On the basis of our previous studies, time-dependent distribution, protein and gene expression of cannabinoid type 2 receptor (CB2R) are investigated in an animal model of mouse skin incised wound and cultured fibrocytes, including myofibroblats differentiated from fibrocytes by immunofluorescence and molecular biological techniques in the project. Further studies are conducted on the effect of CB2R on transdifferentiation of fibrocytes to myofibroblasts, regulation for MMP-2 and collagen production,and cell apoptosis as well as related signal pathway by administration of selective CB2R agonist (JWH-133) and antagonist (AM-630) in vivo and in vitro for interpreting the roles of CB2R in skin wound healing. The data obtained will provide further insight into the molecular mechanisms of the cannabinoid receptor in regulation of skin wound repair and valuable information for research and development of drugs targeted to the abnormal tissue wound repair.