70kDa热休克蛋白（Hsp70）是真菌细胞响应压力的关键蛋白。随着Hsp70新的亚型被发现，Hsp70的新功能也被逐渐发掘，但对其作为与DNA结合的转录因子功能目前知之甚少。栓菌AH28-2基因组编码5个Hsp70，其中ThHspA1和ThHspA7可能作为DNA结合蛋白，分别参与邻甲苯胺和愈创木酚压力下的漆酶lacA表达调控。本项目拟以栓菌AH28-2的5个Hsp70为研究对象，选择多种类芳香化合物，诱导漆酶lacA表达，并通过分子生物学等技术，探明在所选化合物暴露下是否均有Hsp70调控lacA表达，不同条件下参与调控的Hsp70类型与化合物类型/结构之间是否存在联系；明晰Hsp70通过何种方式、识别何种DNA序列进而参与调控漆酶lacA表达。研究结果有助于深入理解Hsp70参与调控基因表达的功能，并为揭示芳香化合物压力下漆酶基因差异表达调控机制研究提供新线索。

The 70 kDa heat-shock proteins (Hsp70s) are central components, which are mainly expressed in response to different cellular and environmental stresses in fungal cells. Hsp70s assist a wide range of folding processes, including the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins, etc. However, little information is gained about its role as a DNA binding transcriptional activator of fungal genes in response to extracellular stresses. Previously, we found that two of the five Hsp70s (designated as ThHspA1 and ThHspA7) in Tramets sp. AH28-2 may act as DNA binding proteins and are involved in regulation laccase (lacA) expression during induction with o-toluidine and guaiacol, respectively. In order to know more about Hsp70s, in this proposal, we plan to induce lacA production with more types of phenolic compounds, to elucidate the relationship between Hsp70 expression and the types/characteristics of phenolic compounds. For example, whether there is always a type of Hsp70 acts as a DNA binding regulator to regulate lacA expression after phenolic compound exposure? Then, we will study on the interaction between the Hsp70s and their binding sequences through DNA footprinting, EMSA, and protein crystallography technique, etc, to elucidate the mechanism by which the Hsp70s regulate the expression of lacA. The results are supposed to improve our understanding of Hsp70 that acts as a DNA binding protein, and will provide some clues on exploring the mechanism of differencial laccase regulation.