内质网是真核细胞中结构复杂，且高度动态的膜性细胞器，担负着蛋白合成和加工、脂质合成及维持胞内钙离子浓度稳定等多种功能。然而，对于内质网形态的形成机制，特别是在各种因素刺激下所发生的结构动态变化的机制仍是一个谜。在本项目中，将从已知的内质网形态结构相关蛋白入手，筛选新的与片层状内质网和管状内质网的形态变化相关的蛋白，分析其与细胞骨架和线粒体等不同细胞器之间的关系，阐明调控内质网结构动态变化的分子机制。此外，我们前期结果显示，不同诱导因素引起的细胞凋亡过程中，一些内质网结构蛋白如REEP1/2发生变化各异，从而调控内质网形态结构，进而调控细胞凋亡进程。在本项目中，我们将深入探讨内质网结构动态变化与细胞骨架相关组分的关系，以及其响应细胞凋亡信号发生形态变化的信号通路，揭示不同诱导因素引起的细胞凋亡条件下内质网形态动态变化过程及其调控的分子机制，为相关疾病的治疗提供理论依据。

The endoplasmic reticulum (ER) is one of the most complicated and dynamic membrane-bound organelles in the eukaryotic cell. It plays a critical role in many cellular processes, including protein synthesis and modification, lipid synthesis and the regulation of Ca2+ homeostasis. However, it is still mysterious how ER structure changes, especially under various stress conditions. In this project, we will screen and analyse the protein components involved in the formation of the ER sheets and ER tubules, investigate the relationship between ER and various organelles, such as cytoskeleton and mitochondria, and understand the functions and molecular mechanisms of ER shaping. Moreover, our preliminary data show that the structure of the ER changes dramatically during apoptosis induced by various stimuli. Some ER shaping proteins, such as REEP1/2, are involved in this process and could affect apoptosis process vice versa. Therefore, we will investigate the relationship between dynamics of ER structure and cytoskeleton, and the signal pathways underlying the apoptosis-triggered ER sturcture change in depth. We will also reveal the molecular mechanisms of ER dynamics and functions during the process of apoptosis, and provide theoretical foundations for clinical applications.