基于FePt/GO纳米复合材料诊疗探针：构建、多模成像和抗肿瘤活性研究

本项目以FePt/GO纳米复合材料为研究对象，设计集多靶向（分子水平+外磁场）、双模成像诊断（MRI+荧光）和双重治疗（化疗+热疗）一体的诊疗功能纳米探针。从分子、细胞和组织三个层次系统研究FePt/GO诊疗探针对肿瘤多靶向、多模成像和治疗的可行性、选择性和特异性,提高微小病灶的检出率，获得肿瘤治疗及疗效评价的可视化信息。通过石墨烯表面耦联荧光素,结合FePt的超顺磁性，实现对肿瘤细胞的双模可视化，通过选择不同肿瘤靶点（叶酸受体等），获得对肿瘤细胞分子水平上的靶向选择性。研究pH值响应的FePt"化疗"和石墨烯"热疗"双重治疗效果，研究FePt在肿瘤细胞内诱导肿瘤细胞凋亡机制。 通过对本项目的实施，获得对肿瘤能够实现双靶向、双模成像诊断、双重治疗的特异性诊疗功能纳米探针，实现治疗及疗效评价的实时监控，为构建新型抗肿瘤纳米药物诊疗平台提供新方法与新途径。

Based on the FePt/GO nanocomposites, this project aims to design the theranostic nanoprobe with dual targeting(molecular + magnetic), dual modal imaging diagnosis(MRI + fluorescence) and simultaneously dual therapy(chemotherapy + photothermal). We research the feasibility, selectivity and sensitivity of the mulitfunctional FePt/GO theranostic nanoprobe for tumor targeting multimodal imaging diagnosis and in situ therapy from the molecular, cell and tissue levels, to improve the detection rate of tiny lesions and obtain the visual information of diagnosis and therapy.Through coupled with fluorescein and the superparamagnetic FePt nanoparticles on graphene oxide surface, we realize the dual-modal visualization on tumor cells (MRI-optical imaging); by choosing different tumor targets (folic acid receptors,etc), the molecular level targeting selectivity of tumor cell has been obtained. Based on pH- responsive FePt "chemotherapy" and GO "photothermal therapy" dual effects, we expect to obtain the apoptotic mechanism of tumor cells. Through the implementation of the project, we expect to obtain the theranostic nanoprobes for dual-modal imaging diagnosis and synchronous therapy, to implement the real-time monitoring of diagnosis and therapy evaluation, to provide new methods and ways for the building a new anti-tumor drug theranostic platform.