贵金属纳米粒子自组装表现出结构相关联的强光学特性，等离子相互作用呈现整体放大效应。纳米四面体拥有独特结构及良好的生物反应活性和生物相容性使其在生物标志物传感分析方面具有极大的优势。本项目拟进行纳米粒子可控合成、适配体核酸骨架设计及纳米粒子界面修饰，实现功能性纳米探针制备。基于分子识别纳米材料自组装技术，构筑银纳米粒子四面体及其与蛋白共组装结构，进行动态组装过程研究。开展纳米粒子四面体及其与蛋白共组装体的不同结构构型和光谱学关联及机制研究；建立光学模型，阐释纳米四面体拉曼增强效应。进行纳米粒子四面体对生理溶液稳定性修饰，构建基于表面增强拉曼散射光谱的癌症标志物高灵敏、多重检测方法，为多种癌症标志物同时测定的医学检测器件构筑提供新的思路。

Noble nanoparticles assemblies showed structure dependent strong optical properties, in which Plasma interaction exhibited collective amplification effect. Nanoparticle pyramids possess unique structures, good biological reactivity and biocompatibility that have a great advantage in the sensor detection of biomarkers. This project will perform nanoparticle controllable synthesis, aptamer DNA skeleton design, nanoparticle interface modification to achieve functional nano-probes fabrication. Through the molecular recognition based nanoparticle self-assembly technology, this project will construct silver nanoparticle pyramids and the pyramids with proteins co-assembly structures, and investigate the dynamic assembly process. This project will also carry out the relationship and mechanism study between optical properties and different configuration structures of nanoparticle pyramids and its proteins co-assembly structures. The optical models will be established and Raman enhancement effect of nanoparticle pyramids will be demonstrated. The nanoparticle pyramids will be modified to improve stability against physiological solution, then the surface enhanced Raman scattering spectra based high sensitive and multiple cancer markers detection method will be constructed. This project provide new ideas to build medical sensor devices for simultaneous determination of multiple cancer markers.