豆类中黄酮类化合物与蛋白/肽具有协同调节脂代谢作用，但二者相互作用及协同介导脂交换的机制尚不清楚。本项目以鹰嘴豆芽素 A（biochanin A）和肽CPe-III为研究对象，拟利用紫外光谱、核磁共振、分子模拟等技术探究二者之间的相互作用规律和关系；通过细胞培养、动物模型体系从生理指标、脂代谢相关的基因和蛋白表达水平的角度揭示二者协同调节脂代谢的分子生物学机制；以胆固醇酯转运蛋白(CETP)为作用靶点，采用分子对接及分子动力学理论解析biochanin A、CPe-III及biochanin A-(CPe-III)复合物与靶点CETP蛋白分子间结合模式、作用机制；从而深入探讨鹰嘴豆膳食干预脂代谢的构效关系。研究结果为食品中组分(尤其是黄酮与蛋白/肽之间)相互作用研究提供新的思路与借鉴，对鹰嘴豆资源的充分利用和促进人类健康都具有科学意义和学术价值。

Flavonoids and protein/peptide in beans show synergistic effect in regulating lipid metabolism, while the mechanism of interaction and collaborative-mediated fat exchange pathway are still unclear. In this project, ultraviolet spectrum , NMR and molecular simulation technology will be used to explore the relationship of biochanin A and peptide CPe-III. The function and mechanism of regulating lipid metabolism based on biochanin A and peptide CPe-III will be illustrated by the physical index , expression levels of proteins and genes in vitro and in vivo. Meanwhile, molecular docking and molecular dynamics theory will be applied in exploring the combination mode of biochanin A, CPe-III and their complexes targeting on cholesterol ester transfer protein (CETP) as well as the structure-activity of fat metabolism. In conclusion, all the results will provide referential experience and new ideas for exploring the mechanism of multicomponent in food (especially between flavonoids and protein/ peptide). The results will also provide the theoretical foundation and science basis for the comprehensive utilization of chickpea resources and development the human health.