目前突破肿瘤诊疗效果难点的关键是发展多模态、多用途分子影像探针。然而现有多模态分子影像探针大多采用各成像组件通过表面键合组装而成，往往造成探针性能不佳等问题。本项目采用全新思路,以I-III-VI族元素和Gd元素为原料，在量子点合成过程中即引入顺磁性Gd离子，合成出高质量顺磁性量子点（pQDs）；另考虑pQDs的生物适配性，提出一种有效的量子点转相方法，在超声作用下利用生物蛋白一步法转相量子点，解决当前量子点的非特异性吸附问题，有效减弱血液调理作用和RES捕捉；再偶合肿瘤靶向配体，构建出全新模式的生物适配型、荧光效率高、弛豫性能好和靶向性强的新型双模态成像探针。从而实现对原位肝癌非侵入性的连续动态双模态成像。并在此基础上，借助探针的近红外荧光导航功能，术中实施肿瘤和微小转移病灶的切除和清扫，力求解决目前肿瘤手术切除不彻底而易复发的难题。本项目将为原位肝癌的早期诊断和手术精准切除提供新策略。

The current challenges in early cancer treatment lie on the development of versatile multi-modal probes that can enable clinical real time imaging-guided surgery. However, the current synthesis of the probe materials is limited by simple assembly of individual imaging moieties leading to interference among various functional components. In this proposed study, paramagnetic quantum dots (pQDs) will be developed by introducing paramagnetic Gd ion to the I-III-VI element - based quantum dots during the synthesis. For biocompatibility of pQDs, an effective phase-transfer method will be developed. Under the condition of ultrasound, QDs can be water-solublized with biological proteins. This one-step phase-transfer method can solve the problem of non-specific adsorption of QDs, and effectively weaken blood opsonization and escape RES capture. Upon conjugating with tumor targeting ligands, a new type of bimodal imaging probe is designed and constructed with good biocompatiblity,high fluorescent quantum yield,high relaxation rate, and strong targeting ability for non-invasive continuous dynamic imaging of orthotopic liver tumor. To effectively reduce tumor recurrence caused by incomplete tumor surgical resection, the NIR fuctionality of probe will be employed to distinguish tumor sites and sentinel lymph node during imaging-guided surgery. This project will provide a new strategy for early diagnosis and surgical precision resection of orthotopic liver cancer.