新疆少数民族人群39个STR基因座遗传多态性及族源关系研究

目前STR分型技术已广泛应用于个人识别和亲权鉴定中，但有关新疆少数民族人群STR的群体调查资料还很缺乏，以汉族群体资料为依据计算得出的鉴定意见，在上述民族案件的诉讼中日益受到质疑，因此迫切需要完善新疆主要少数民族常见STR基因座的群体遗传资料。本课题拟在前期工作基础上采集维、哈、回、柯尔克孜族无血缘个体口腔拭子各1000人份，利用荧光标记检测系统对39个常染色体STR 基因座进行检测并获得频率分布，根据检测结果建立四个民族39个基因座的多态信息库，并计算各基因座的杂合度(He)、个人识别能力(DP)、偶合率(PM) 和非父排除率(EP)等群体遗传学参数，评价其法医学应用价值，以期完善上述群体遗传学的数据资料，为新疆少数民族地区法庭科学应用提供可靠的基础数据；同时进一步将所得数据在国内外不同群体之间进行比较，探讨群体间是否存在遗传差异，为民族起源、迁徙等其他研究提供一定分子生物学理论依据。

The short tandem repeat (STR) STR typing technique currently have been widely used in the individual identification and paternity test. However, a STR population survey data on ethnic minorities in Xinjiang area has not been well-established. It is therefore increasing questioning for the case identification report based on population survey data from Han nationality for the above mentioned nationality. So it is really required to establish the common STR loci for ethnic minorities population. This proposal intends to collect 1000 oral swabs from unrelated Uygurs,Kazak,Hui and Khalkhas population, and the distribution frequencies of STR loci in total of 39 autosome are tested with GlobalFiler? and AGCU kit fluorescent labeling system. And the population genetic data including the heterozygosity(He) of STR loci, discrimination power (DP),Matching probability（PM） and exclusion probability (EP) are calculated to evaluate its potential medical value. The proposal will establish the population genetic data for Uygurs ,Kazak，Hui and Khalkhas nationality, which will provide sound and reliable basic population genetic data for Forensic Science in Xinjiang minority areas. This proposal will further provide molecular biological basis for the study of ethnic origin and migration for ethnic minorities population following the comparison of allele frequencies among diffrent nationality.