三磷酸腺苷双磷酸酶（Apyrase）不仅能水解能量物质ATP，还能控制植物细胞外ATP浓度从而参与一系列信号转导过程，该酶对植物生长发育有重要的调控作用，另一方面植物根的生长发育也会受到介质应力和渗透压力调控。本项目基于实验室长期植物生物力学的研究积累，结合力学、细胞及分子生物学方法，建立可控应力及渗透压交互作用加载模型，以拟南芥APY1-7 敲除, APY1-7 过表达, APY:GUS, APY:GFP等突变体为研究资源，分析植物根系形态、形态结构建成的时空变化规律，以及Apyrase、胞外ATP、细胞骨架等参数与应力之间的变化规律，探讨Apyrase调控的ATP信号及下游信号转导在植物根系生长发育中的作用机制，并建立相应的力发育模型。本研究将进一步揭示植物根系生长发育的调控因素及其信号转导的机制，其研究将对农业增产、生态保护、植物能源等方面具有重要现实意义。

Apyrase can not only hydrolyze ATP which acts as energy substance, but also can control the concentration of extracellular ATP to involve in a series of signal transduction. Apyrase has significant regulating in plant growth and development. On the other hand, plant growth and development is regulated by mechanical stress and osmotic pressure from medium. Based on long-term research of plant biomechanics in our lab, the project could establish a loading model of interaction between controllable stress and osmotic pressure through combining with technologies in mechanics, cytology and molecular biology. Using mutants of Arabidopsis thaliana as the important study resource, such as APY1-7 knockout , APY1-7 over expression, APY:GUS, APY:GFP, we will focus on the rule of root structure’s change, the rule between Apyrase, extracellular ATP, cytoskeleton and stress, and investigate the role of Apyrase and eATP and their downstream signal transduction in root development, therefore build a model of mechanical stress-root development. The result could enrich comprehension of factors regulating plant growth and development, and of signal transduction. The result will contribute to development of agricultural production and plant energy.