鱼类脂肪肝是一种描述鱼类肝脏肝脂过量蓄积为特征的生理现象及其相关代谢性疾病的统称，目前已成为严重困扰水产养殖业持续健康发展的难题之一。在国家自然科学基金的资助下（31402281，已结题），申请者发现ATGL和G0S2是鲤鱼脂肪水解与蓄积的关键因子。在此基础上，本项目以鱼类脂肪细胞的成因作为切入点，通过细胞转染获得Tet1基因过表达、Tet1基因敲除两种鲤鱼前体脂肪细胞，同时建立Tet1基因过表达、Tet1基因敲除两种鲤鱼模型，研究Tet1介导的DNA去甲基化在鲤鱼前体脂肪细胞成脂分化中的作用及调控机制，为揭示鲤鱼脂肪肝形成的分子机制提供科学依据，并为鱼类脂肪肝的早期诊断与养殖的风险预警提供准确的分子标记。

Fatty liver is one kind of physiological phenomenon characterized by excessive fat accumulation in the liver of fish and a general term for its related metabolic diseases, which has become one of popular problems, impeding the sustained and healthy development of the aquaculture industry. Sponsored by National Natural Science Foundation of China (NO.31402281, completed), we have found that ATGL and G0S2 are both the key factors of triglyceride metabolism in Cyprinus carpio. Base on the results, the project will focus on the formation mechanism of lipocyte in fish. We will obtain the cells with Tet1 overexpressed in preadipocytes and Tet1 knocked out in preadipocytes by cell culture and transfection; we also will establish the model of Tet1 overexpressed and Tet1 knocked out in Cyprinus carpio. This study will elucidate the function and regulatory mechanism of adipogenesis differentiation of preadipocyte in Cyprinus carpio by Tet1 on DNA demethylation. The study will provide the scientific results for the formation mechanism of fatty liver in Cyprinus carpio. Moreover, it will provide the accurate molecular marker for the diagnose of fatty liver in fish and the risk alarm in aquaculture industry.