鱼类营养性脂肪肝是脂肪代谢障碍引起的疾病，目前已成为严重困扰水产养殖业持续健康发展的难题之一。最新研究表明，脂肪甘油三酯脂肪酶（ATGL）是脂肪分解限速酶，水解甘油三酯为甘油二酯和游离脂肪酸，该过程是脂肪分解的第一步骤，因此对ATGL及其调控因子的研究有望取得揭示鱼类营养性脂肪肝形成机制的突破。本项目针对调控脂肪分解的ATGL及其关键因子CGI-58和G0S2展开研究，对鲤鱼通过正常投喂、饥饿和饥饿后重新喂食等不同营养条件处理；同时，通过腹腔注射和离体培养肝脏细胞孵育，采用多种脂肪代谢调节剂干预ATGL、CGI-58、G0S2的表达，系统研究鲤鱼ATGL基因和调控因子CGI-58、G0S2在肝脏中的调控作用及其与甘油三酯代谢的关系，以期揭示鲤鱼肝脏中甘油三酯代谢的分子机制，为鲤鱼营养性脂肪肝的防治奠定理论基础和科学依据。

Fatty liver is a nutritional disease caused by lipodystrophy, which has become one of popular problems, impeding the sustained and healthy development of the aquaculture industry. The latest study showed that adipose triglyceride lipase (ATGL) as the lipolysis rate-limiting enzyme could catalyze the initial step of lipolysis converting triglyceride (TG) to diacylglycerol (DG) and free fatty acid (FFA). This process is the first step of lipolysis. Thus, the research of ATGL and its regulatory factors will unveil the mechanism of formation of nutritional fatty liver in fish. The project is focus on the key factors of TG metabolism, which include ATGL and the regulator: CGI-58, G0S2 in the liver of Cyprinus carpio. The pivotal research will investigate as following two fields: different nutritional conditions including feeding, fasting and refeeding period; through peritoneal injection and primary cell culture of liver, we will use a variety of lipid metabolism regulator in order to interfere with the expression of factors, and then concentrate on the systematic investigation, including the regulation pattern of ATGL, CGI-58, G0S2 in the liver and the relation of TG metabolism. This study will elucidate the molecular mechanism of TG metabolism in the liver of Cyprinus carpio. Moreover, it will provide the theory basis and scientice results for the control of nutritional fatty liver in Cyprinus carpio.