以痕量海洋赤潮藻毒素局域表面等离激元共振（LSPR）免疫在线检测需求为牵引，本项目拟发展成本低、工艺可控的纳米复合结构单元二维阵列组装技术及纳米结构生物特异性修饰技术。项目突破纳米LSPR传感基片的常规选择，通过引入过渡层、化学处理及表面修饰等手段，基于光纤构建了可防氧化的纳米复合阵列体系，并引入间接竟争压抑测定法，提升传感器LSPR响应的选择性、精确性及稳定性；构建多条光纤通道的LSPR免疫检测系统，实现三种典型的痕量赤潮藻毒素的快速、同步、在线检测。项目为制造高灵敏度、宽选择性、重复性好的新一代纳米生物传感器提供可靠的工艺技术和科学依据，且为海水水质污染物在线检测提供新的思路。

To satisfy trace and online detection for marine algal toxins using localized surface plasmon resonance (LSPR) immunoassay method, the assembly technology of two-dimensional (2D) hybrid nanostructure array with low-cost and process controllability would be in-depth studied and modification technology of specific biofilm on nanostructure would be well developed. According to intermediate layers introduction, chemical processing and surface modification methods instead of conventional sensing substrate, the antioxidant hybrid nanostructure array film would be fabricated on fiber LSPR sensor. In additional, competitive inhibition immunoassay would employed to promote its selectivity, accuracy and stability. Finally, rapid, simultaneous and online detection for three typical trace algal toxins would be performed via multi-fiber LSPR immuo-detection system. The research could provide processing technology and scientific proof of novel nano biosensor characteristic of high sensitivity, broad selectivity and excellent repeatability. Furthermore, it would help to offer novel thought for noline detection of marine pollutant.