干旱是严重影响农业生产的环境因子之一，干旱胁迫信号转导机制的研究对于培育抗旱植物具有重大意义。一氧化氮（NO）是植物响应逆境胁迫的重要信号分子，蛋白质巯基亚硝基化修饰是一种典型的氧化还原依赖的翻译后修饰。植物不定根发生问题是植物无性繁殖和工厂化育苗实践的核心问题，也是植物发育和形态建成等方面的重要理论问题。我们前期研究已探讨了干旱条件下万寿菊不定根发生中NO和其它信号分子的互作及其生理生化机制。本研究拟采用双向电泳实验、生物素转化法、免疫共沉淀技术结合质谱分析、酵母双杂交系统以及高效液相色谱等手段证明干旱条件下万寿菊的生长素受体TIR1能被巯基亚硝基化修饰，该修饰能调控TIR1和Aux/IAA蛋白的互作，激活下游通路，进而调控万寿菊不定根的发生。拟探讨生长素受体TIR1亚硝基化在干旱条件下不定根发生中的作用及其分子机理，进而揭示干旱条件下NO调控植物不定根发生中蛋白质翻译后修饰的分子机制。

Drought stress is one of the main problems affecting agricultural production, and understanding the signal transduction mechanisms behind plant tolerance and stress response will help us to develop new means of strengthening fruitful agronomy. Nitric oxide (NO) is one of the important signal molecules in plant response to stress. Protein S-nitrosylation is a redox-based post-translational modification of thiol residues. Adventitious root is an important issue both on the theoretical aspect of plant development and organogenesis and on the practical side of plant propagation and excised issue or organ regeneration. Our previous study revealed the signal interactions between NO and other signal molecules during rooting in marigold under drought stress and the interactive mechanisms. On the basis of our previous studies, two-dimensional electrophoresis, biotin switch assay, Co-immunoprecipitation with mass spectrometry, yeast two-hybrid analysis and high performance liquid chromatographic analysis will be used to study the protein S-nitrosylation of the auxin receptor TRANSPORT INHIBITOR RESPONSE 1 (TIR1) in marigold under drought stress in the current study. The S-nitrosylation will regulate the TIR1–Aux/IAA interaction, subsequently promote activation of signaling pathways downstream, and finally regulate the formation of adventitious roots in marigold. These results will underline the roles of the protein S-nitrosylation of the auxin receptor TIR1 during rooting under drought stress and its molecular mechanism. Our findings will illustrate a mechanism by which NO signaling and protein post-translational modification coordinate adventitious root development under in plants drought condition.