不定根途径是园艺植物和林木生产中苗木快速繁育的重要方法，对农、林和园艺业的发展具有重要经济价值。氢气和一氧化氮在植物中的信号分子功能研究是当前国际生物学界的热点。我们前期研究发现氢气和一氧化氮均能诱导植物不定根发生，但两者的信号互作及其机制尚不清楚。本研究拟以万寿菊（Tagetes erecta L.）为试材，运用形态解剖学和药理学的方法系统观察和分析一氧化氮在氢气诱导万寿菊不定根发生中的信号功能；在此基础上，采用生理学、细胞生物学和分子生物学的方法研究一氧化氮参与氢气诱导不定根发生过程中细胞周期时相、抗氧化酶系统、内源氢气和一氧化氮以及生根靶基因表达等的变化，以揭示不定根发生中一氧化氮参与氢气信号过程的生理生化和分子生物学机制。为氢气和一氧化氮信号调控植物生长发育研究补充新资料，拓展对植物不定根发生调控机制的认识，以期为氢气和一氧化氮在不定根发生等农业生产实践中的应用提供新资料和新思路。

The formation of adventitious roots is a common vegetative propagation practice in forestry, horticulture, and fruit crops, which is a complex genetic process regulated by both environmental and endogenous factors. Adventitious root formation has many practical implications in horticulture and agronomy and there is a lot of commercial interest.It has now become well accepted that hydrogen and nitric oxide may have key roles to play in the signalling that takes place in plant cells. The signal roles of hydrogen and nitric oxide in plants become a hot topic in the academic community of the biological study. Our previous results found that both hydrogen gas and nitric oxide may induce adventitious root development in plants. However, the signal interation between them and its mechanism involved are not elucidated. On the basis of our previous studies, the signal roles of nitric oxide in hydrogen-induced adventitious rooting will be observed and analysized systematically by morphological, anatomical and pharmacological studies. In addition, with the methods of physiology, cell biology and molecular biology, the changes of cell cycle phase, antioxidant enzymes system, endogenous hydrogen and nitric oxide, and representative target genes related to adventitious rooting will also be studied, in order to reveal the mechanism of biochemistry and molecular during hydrogen-induced adventitious rooting involving the nitric oxide pathway. Expected results of the project will provide some academic reference to the study of plant growth and development regulated by hydrogen and nitric oxide, expand and enrich the knowledge of the mechanism of adventitious root formation in plant, and also provide theoretical basis for the application of hydrogen and nitric oxide in agricultural production.