Abscisic Acid Induces Adventitious Rooting in Cucumber (Cucumis sativus L.) by Enhancing Sugar Synthesis.
Abscisic Acid Induces Adventitious Rooting in Cucumber (Cucumis sativus L.) by Enhancing Sugar Synthesis.
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脱落酸通过增强糖合成诱导黄瓜 (Cucumis sativus L.) 不定根
DOI：
10.3390/plants11182354
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发表时间：
2022-09-09
期刊：
影响因子：
--
通讯作者：
Liao W
中科院分区：
文献类型：
--
作者：
Li C;Zhang M;Qi N;Liu H;Zhao Z;Huang P;Liao W
脱落酸（阿坝）影响植物的许多重要生理过程，如种子萌发、根伸长和气孔运动。然而，阿坝和不定根形成过程中的糖合成之间的关系的信息很少。本研究的目的是评估阿坝对黄瓜不定根形成的影响，以及这种植物激素对糖合成的影响是否可以包括作为不定根发育的一个致病因素。测定了阿坝处理下葡萄糖、蔗糖、淀粉、总糖含量及蔗糖合成酶（SS）、蔗糖磷酸合成酶（SPS）、己糖激酶（HK）和丙酮酸激酶（PK）活性。我们还定量了在此过程中蔗糖或葡萄糖合成基因的相对表达。增加阿坝浓度显著促进不定根的形成，以0.05 μM浓度效果最显著。与对照相比，阿坝处理的葡萄糖、蔗糖、淀粉和总糖含量均有所增加。阿坝处理提高了黄瓜不定根发育过程中葡萄糖-6-磷酸（G6 P）、果糖-6-磷酸（F6 P）和葡萄糖-1-磷酸（G1 P）的含量，并提高了蔗糖相关酶SS和SPS以及葡萄糖相关酶HK和PK的活性。与此同时，阿坝上调蔗糖或葡萄糖合成相关基因的表达水平，包括CsSuSy 1，CsSuSy 6，CsHK 1和CsHK 3。这些结果表明，阿坝可能通过提高葡萄糖、蔗糖、淀粉、总糖、G6 P、F6 P和G1 P含量、SS、SPS、HK和SPS活性以及CsSuSy 1、CsSuSy 6、CsHK 1和CsHK 3基因的表达水平，促进不定根的发育。这些发现为阿坝在不定根形成过程中的生理作用提供了证据，并为阿坝与植物不定根过程中糖合成的可能关系提供了新的认识。
Abscisic acid (ABA) affects many important plant processes, such as seed germination, root elongation and stomatal movement. However, little information is available about the relationship between ABA and sugar synthesis during adventitious root formation. The aim of this study was to evaluate the effect of ABA on adventitious root formation in cucumber and whether the effect of this plant hormone on sugar synthesis could be included as a causative factor for adventitious root development. We determined the contents of glucose, sucrose, starch, total sugar and sugar-related enzymes, including sucrose synthase (SS), sucrose phosphate synthase (SPS), hexokinase (HK) and pyruvate kinase (PK) activities in ABA treatment. We also quantified the relative expression of sucrose or glucose synthesis genes during this process. Increasing ABA concentrations significantly improved adventitious root formation, with the most considerable effect at 0.05 μM. Compared to the control, ABA treatment showed higher glucose, sucrose, starch and total sugar contents. Moreover, ABA treatment increased glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P) contents in cucumber explants during adventitious root development, which was followed by an increase of activities of sucrose-related enzymes SS and SPS, glucose-related enzymes HK and PK. ABA, meanwhile, upregulated the expression levels of sucrose or glucose synthesis-related genes, including CsSuSy1, CsSuSy6, CsHK1 and CsHK3. These results suggest that ABA may promote adventitious root development by increasing the contents of glucose, sucrose, starch, total sugar, G6P, F6P and G1P, the activities of SS, SPS, HK, SPS and the expression levels of CsSuSy1, CsSuSy6, CsHK1 and CsHK3 genes. These findings provide evidence for the physiological role of ABA during adventitious root formation and provide a new understanding of the possible relationship between ABA and sugar synthesis during adventitious rooting in plants.
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影响因子：
5.1
作者：
Dekkers, Bas J. W.;Schuurmans, Jolanda A. M. J.;Smeekens, Sjef C. M.
通讯作者：
Smeekens, Sjef C. M.
蔗糖和脱落酸对玉米胚乳淀粉合成相关基因的协同影响
影响因子：
3.1
作者：
Chen, Jiang;Huang, Binquan;Huang, Yubi
通讯作者：
Huang, Yubi
影响因子：
4.2
作者：
Guo, Dongliang;Liang, Jianhua;Li, Ling
通讯作者：
Li, Ling
影响因子：
6.9
作者：
Liu Y;Ye N;Liu R;Chen M;Zhang J
通讯作者：
Zhang J
影响因子：
9.4
作者：
Loreti, Elena;Povero, Giovanni;Perata, Pierdomenico
通讯作者：
Perata, Pierdomenico