大豆是我国食物蛋白和饲料蛋白的主要来源之一。大豆中所含大豆异黄酮（SIF）能与动物体内雌激素受体结合并产生多种类雌激素的作用。实验动物的研究表明，大量摄入SIF严重干扰雌性鼠的繁殖内分泌功能，但其对家畜繁殖生理是否有相似的影响迄今鲜见报道。因此，研究SIF对家畜繁殖生理的影响具有重大的学术意义和广阔的应用前景。本项目拟以母猪为研究对象，运用生化技术、放免技术、RT-PCR技术、Nothern blot技术、流式细胞技术和电镜技术等手段，研究SIF对母猪生殖激素分泌、卵巢发育、卵子生成和卵巢组织生化功能和卵巢内生殖细胞凋亡的影响，揭示SIF对母猪卵巢和大脑相关酶活性及基因表达的调控作用，探索SIF对母猪下丘脑-垂体-性腺轴的影响，进而阐明SIF作用于母猪生殖生理的分子机制。为推进SIF的理论研究,评价大豆食用和饲用的安全性,提高畜禽的繁殖效能，确保人类合理、安全利用大豆产品提供科学依据。

In China, soybean is one of the main sources for people to obtain food protein and feed protein. The soy isoflavones (SIF) contained in soybean can integrate into the animal estrogen receptor, and help producing different kinds of estrogens. .Studies on Lab. animals have proven that a high-intake SIF can interference the reproductive endocrine function on female mice seriously, but rare report proves that there is a similar interference on livestock reproduction so far.Therefore, research on the effects of SIF on livestock reproduction has a great academic significance and it will turn out a extensive applied foreground..This plan is using sow as the experimental subject. By using biochemical technology, RT-PCR, Nothern Blot, flow cytometry technology and electron microscope term methods, we can find out how the SIF affects reproductive hormone secretion, development of ovary, eggs generation, ovarian tissue biochemical functions, apoptpsis of reproductive cells in the ovary, in order to reveal the regulatory function of SIF on sow ovarian, the related enzymes in brain, and the gene expression. Further more, it will explore the effects of SIF on sow's gonadal axis, and illustrate the SIF role in the molecular mechanism of reproductive physiology of sow.We will provide scientific basis to promote the study of the theory of the SIF, evaluate the safety of soybean to be used in food and forage, improve breeding efficiency of livestock and poultry, and ensure human to use soy beam products reasonably and safely.