前期研究发现健脾益肾强骨针法能够增强股骨的弹性和硬度，预防骨质疏松性髋骨骨折的发生,但研究结果表明针刺对股骨力学性能的影响不能完全解释针刺对在体股骨抗外力性能的增强。预实验表明针刺能够改变肌纤维结构，增强肌肉力量。为进一步证实健脾益肾强骨针法不仅能通过影响股骨的力学性能，同时还能改变肌肉的力学性能，来增强在体股骨的抗外力能力，从而预防骨质疏松性髋骨骨折的发生。拟以雌性大鼠去势术造模，检验造模成功后,以足三里、肾俞、大杼为主穴，手法为捻转补法的健脾益肾强骨针法治疗,通过检测在体股骨和离体股骨的最小骨折外力,分析肌肉在骨折发生瞬间所起的缓冲和减小股骨最终受力作用, 研究在骨质疏松疾病状态下针刺对肌肉生物力学性能的影响,并从微循环血流灌注量和肌纤维微结构两方面深入探讨针刺对股四头肌生物力学性能产生影响的可能内在机制，揭示针刺预防骨质疏松性髋骨骨折的作用机制，并为骨质疏松性骨折的预防提供新的思路。

•.According to the previous study we found that acupuncture could enhance thigh bone’s hardness and elasticity. But the study shows that the acupuncture effect of thigh bone’s mechanical property couldn’t explain how the external force resistance increased. The prepare experiment shows that acupuncture could transform the micro structure of muscle fiber and enhance the muscle force. To confirm that acupuncture could enhance mechanical property both of thigh bone and muscles. So that it can prevents the hip from osteoporotic fracture by enhance the ability of anti-external force of vivo thigh bone. We Intends to do acupuncture at BL23, BL11 and ST36 of the ovariectomized rats. By testing the minimum force which cause the vivo and in vitro thigh bones broken, to study about the acupuncture effect of muscle biomechanical property. And discuss the inner mechanisms further as following : microcirculation blood perfusion volume and micro structure of muscle fiber. To reveal the mechanisms of preventing hips from osteoporotic fracture of acupuncture. Provide new idea about preventing osteoporotic fracture.