前期研究发现，经络辨证指导下的逆针灸能够明显降低雌性SD大鼠自发性乳腺肿瘤和DMBA诱发的乳腺癌的发病率和荷瘤数，减缓病理演变过程，调整ER、PR的表达和CA15-3的浓度，表明逆针灸能够调节肿瘤细胞增殖、分化和凋亡。为进一步明确逆针灸在乳腺癌“正常→一般增生→非典型增生→原位癌→浸润性癌”谱带式连续过程中的各个阶段，尤其是癌前病变阶段，其调节肿瘤细胞增殖、分化和凋亡的作用及其可能的作用途径。拟以DMBA诱发乳腺癌演变过程的动物模型，动态观察乳腺组织病理学变化(细胞分化)，观察与乳腺癌生物学行为密切相关的关键分子，以ERα、ERβ、PRα、PRβ、CerbB-2、PTEN、ki67、PCNA间接反映肿瘤细胞增殖情况，以P53、bcl-2及TUNEL法间接反映肿瘤细胞凋亡情况，探讨以足三里、三阴交、膻中为主穴的针刺方法在正常乳腺上皮细胞向恶性转化的渐进过程当中阻止或逆转癌前病变的作用机制。

Abstract:Our preliminary study discoverd that pre-acupuncture under the guidance of the meridian syndrome differentiation could significantly reduce the incidence of breast cancer and tumor-burdened number, slow down the process of pathological evolution of breast cancer. And adjust the expression of ER、PR and the concentration of CA15-3.These show that the pre-acupuncture is capable to effect the proliferation, differentiation and apoptosis. In the process of continuous about breast cancer from normal to general hyperplasia toatypical hyperplasia to carcinoma in situ to invasive carcinoma .Especially atypical hyperplasia of the precancerous stage. In order to further clear the pathways of pre-acupuncture in the process of the continuous . Taking the DMBA induced production conforms to animal models of human breast cancer evolution. Through dynamic observation in different periods of breast tissue pathology change to observe the differentiation of tumor cells. At the same time observe the key molecules that is closely related to breast cancer biology behavior in the present study. With ERα、ERβ、PRα、 PRβ、CerbB-2、PTEN、ki67、PCNA indirectly indirectly reflect the tumor cell proliferation, with P53、bcl-2、TNUEL method indirectly reflect the tumor cell apoptosis. From normal mammary epithelial cells to the malignant transformation of the gradual process, to explore the mechanism of action with the main point of Zusanli、Sanyinjiao、Danzhong to prevent or reverse precancerous lesions.