亚硝酰氢（HNO）和一氧化氮（NO）是人体内重要的内源性活性氮物质（RNS），两者发挥着截然不同的生理功能，同时相互之间又存在着密切的联系。目前报道的大多数荧光探针只含一个识别位点，只能检测单一目标分子，这类探针不适合用于研究复杂生命体系中多个生物活性分子之间的联系。本项目旨在构建分别以萘酰亚胺和苯并噻唑萘为能量供体、罗丹明为能量受体的具有三发射带或双光子发射的FRET荧光分子平台，并基于该平台发展能单独和连续识别HNO和NO的具有多种信号模式的单荧光探针。本项目所发展的HNO/NO荧光探针可以通过三色或双光子比率荧光成像检测细胞和组织的HNO和NO，这对研究生命体系中HNO和NO之间的生理联系和它们的生理功能有重要意义，也有助于探索与HNO和NO相关疾病的发生发展机制以及研发治疗相关疾病的药物。

Nitroxyl (HNO) and nitric oxide (NO) are the important reactive nitrogen species (RNS) in human body. Though they differ in physiologic role, at the same time, there is a very close relationship between them. Most of the existing fluorescent probes contain one recognition site and respond to one special target, which makes them unsuitable for researching the relationship between two or more biologically active molecules in complicated living systems. This project chooses naphthalimide/benzothiazole-naphthalene as the energy donor and rhodamine as the energy acceptor to develop the FRET fluorescent moleculars with three emission bands or two-photon (TP) emission, and then further developing the single fluorescent probe that can respond to HNO, NO, and HNO/NO with multiple sets of fluorescence signals. The HNO/NO probes developed in this project are capable of detecting HNO/NO using three-color or TP ratiometric fluorescence imaging in living cells or tissues, which is significant for studying the physiological association between HNO and NO and their physiological function in living systems. It will also help explore the mechanism of the occurrence and development of HNO/NO-related diseases and develop relevant drugs.