支链氨基酸是唯一在骨骼肌内发生高程度代谢的氨基酸。KLF15是短期饥饿条件下骨骼肌支链氨基酸代谢调控的重要因子。生物钟是昼夜节律产生的物质基础。在短期饥饿过程中是否存在以KLF15为核心节点的钟控通路及其调控肌肉支链氨基酸适应性代谢变化的机制是亟待解决的科学问题。因此我们拟通过分析短期饥饿过程中鳜鱼肌肉组织核心生物钟基因、KLF15和代谢关键酶基因的节律性表达谱，筛选与KLF15节律性表达变化强关联性的核心生物钟基因和代谢关键酶基因，并在细胞和机体水平验证这些基因是否是KLF15上下游钟控关键基因，确定不同饥饿时间段调控肌肉支链氨基酸代谢KLF15钟控通路，揭示在短期饥饿过程中鳜鱼核心生物钟基因、KLF15和代谢关键酶共同精确动态调节肌肉支链氨基酸代谢的调控机制。其研究结果不仅有助于我们更深一步阐明鱼类肌肉适应代谢变化的调控机制，而且将为我国鱼类高效健康养殖提供重要理论依据。

Branched chain amino acids are the amino acids that are highly metabolized in skeletal muscle. KLF15 is an important factor in the regulation of branched chain amino acid metabolism in skeletal muscle during short-term starvation. The biological clock is the material basis of the circadian rhythm. The KLF15 clock control pathway on regulating the branch chain amino acids adaptive metabolic changes in muscle during short-term starvation is an urgent scientific problem to be solved. So we intend to analyze the rhythmic expression profiles of core clock genes, KLF15 and important metabolic enzymes genes in muscle tissue of Chinese perch during short-term starvation, and screen out the core clock genes and important metabolic enzymes genes that are strongly associated with KLF15 rhythmic expression changes, and determine the KLF15 clock controlled pathway at the different starvation time and reveal the control mechanism of the core clock, KLF15 and key enzymes on BCAA metabolism. The research results will not only help us further clarify the regulation mechanism of fish muscle amino acid metabolism, but also provide the technical implications for aquacultural application.