昆虫介体传播植物病毒的过程是病毒粒体与介体相互识别、相互作用的结果，而病毒与介体的蛋白互作决定着介体传毒的特异性和传播效果。烟粉虱Bemisia tabaci半持久性传播的瓜类褪绿黄化病毒（Cucurbit chlorotic yellows virus, CCYV），是一种新的瓜菜类病毒，在我国和亚洲许多国家造成严重危害，但其传播机理的研究刚刚起步，病毒外壳蛋白与介体受体蛋白互作机理的研究尚属空白。本申请项目在前期研究的基础上，利用免疫学、酵母双杂交、激光共聚焦显微镜、膜饲喂技术等研究CCYV与介体烟粉虱的结合机理，内容包括：CCYV病毒外壳蛋白（CP）和次要外壳蛋白（CPm）在病毒与介体结合中的功能，介体烟粉虱受体蛋白的筛选及功能验证等。本项目的研究结果将提供半持久病毒传播机理的实验例证，有助于加深对植物病毒-介体昆虫互作的认识，同时或将为研发控制介体传毒的新技术和新措施奠定基础。

Transmission of plant viruses by insect vectors results from mutual recognition and interaction between virus particles and their vectors. Specificity and efficiency of plant virus transmission depend largely on protein-protein interactions of viruses and vectors. Cucurbit chlorotic yellows virus (CCYV), a newly reported semipersistent virus transmitted specifically by Bemisia tabaci, has caused serious damage on cucurbit and vegetable crops in many Asian countries. However, researches on CCYV transmission mechanisms have just got started in recent years, and especially studies on interactions of virus coat proteins and vector receptor proteins are still lacking. Based on the previous research results on CCYV transmission, the present project, by using techniques of immunology, yeast two-hybrid, laser scanning confocal microscope and membrane feeding, is proposed to elucidate the binding mechanism of CCYV virons with its whitefly vector, B. tabaci. The experiments in the project include binding function verification of CCYV coat protein (CP) and minor coat protein (CPm), screening and function determining of receptor proteins in B. tabaci. The results of the proposed project will hopefully provide experimental evidences for transmission mechanisms of semipersistent plant viruses, help to deepen our understanding of virus-vector interactions, and may lay the basis for development of new technologies and new strategies for effective control of plant virus transmissions.