一个具有新型表型的甘蓝型油菜T-DNA插入突变体雌蕊发育的分子调控机理

雌蕊发育直接关系到作物种子的产量和质量，是世界性的研究热点。然而，油菜等作物雌蕊发育分子调控机理的研究严重滞后，其主要原因是相关突变体的严重缺乏。本项目前期获得了一个具有超长雌蕊表型的甘蓝型油菜T-DNA插入突变体，命名为Bnspt。本项目将研究这个突变体雌蕊发育的分子调控机理，其研究意义如下：⑴Bnspt突变体的超长雌蕊导致了超长角果和超大种子，千粒重增加了101%，因此本研究在提高产量上具有重要的生产意义；⑵Bnspt突变体展现一个新型表型，完全不同于目前研究雌蕊发育所用突变体的雌性不育表型，因此本研究将会获得完全不同于以前的新的发现；⑶本项目已经克隆到了Bnspt基因组T-DNA插入的侧翼序列及其指示的基因，这为本项目奠定了基础，进而发现T-DNA插入了这个基因的3'-UTR区，导致了其显著上调表达，这将涉及转录后调控事件，从这一新的角度去探讨将会揭示植物雌蕊发育的新的调控方式。

The gynoecium development directly concerns the yield and quality of crop seeds and is a research hotspot in the world. However, knowledge about molecular regulation mechanism in gynoecium development of oilseed rape and other crops is lagging far behind, mainly because of the serious lack of the associated mutants. The preliminary work of the project obtained a T-DNA inserted Brassica napus mutant showing super long pistil phenotype and named it as Bnspt. The project will research the molecular regulation mechanism in the gynoecium development of the mutant. Its research significances are as follows: 1) The super long pistil of the Bnspt mutant leads to super long pod and super large seed, and 1000-seed weight increases by 101%, so the project has the important production significance in the improvement of yield. 2) The Bnspt mutant exhibits a new-style phenotype completely different from the female sterile phenotype of mutants presently used in the studies of the gynoecium development, so this project will obtain new discoveries completely different from previous. 3) The preliminary work of the project have cloned the T-DNA flanking sequence and its indicated gene, which lays the foundation for the develop of this project, further found that the T-DNA inserted into 3'-UTR region of the gene and led to significantly up-regulating expression of the gene, which will involve post-transcriptional control events. So the research on this new point of view will reveal a new regulation pattern of the plant gynoecium development.