研究工业生物过程中两个关键技术生物催化剂酶的多酶组装和高效分离与检测技术的功能配基选择中共同基本科学问题：生物分子相互作用和分子识别机制。建立生物分子相互作用和识别的研究方法包括理论计算方法如分子模拟和多种实验方法（多维核磁和共聚焦显微技术等）以及多种方法的相互结合。研究酶与环境分子的相互识别与相互作用机理，特别是典型生物催化剂多酶组装后活性变化与多酶互动协调催化关系，构建高效的可以调控的磁性纳米酶和多酶超分子体系。同时研究生物分子分离和检测中新的基于弱相互作用的超分子构建以及分子识别机理，构建新的混合模式超分子分离介质，为新型超分子构建和机理研究提供方法基础。发表SCI论文 20篇以上，申请国内外发明专利4-5项，举办相关的国际研讨会。

This project focus on the basic scientific principle in self-assemble of multi-enzyme biocatalysis and ligands of chromatographic separation and detection in industrial biotechnology process: the molecular recognition and interaction regulation mechanism between biological molecular and environmental molecular. The research methods include molecular simulations suitable for biological macromolecules, and a variety of experimental design (multi-dimensional NMR and confocal microscopy), as well as the methods combined. The molecular recognition and interaction mechanism of the enzymes and the environment molecular will be investigated, especially the typical biocatalyst enzyme activity, stability and coordination after enzymes self-assembled. Magnetic nanoenzymes and enzyme supramolecular systems which have biological catalyst catalytic specificity and adaptability to environment regulation will be built. Meanwhile, construction of a new mixed mode of supramolecular and molecular recognition mechanism will be carried out to provide the basis methods for related research. This research will publish more than 20 SCI papers, apply for 4-5 patents and hold related conferences.