结核分枝杆菌细胞壁对维持其形态完整性、抵御化学物质的侵蚀、免疫逃逸等都起着重要的作用。因此，细胞壁合成基因的高通量筛选是研发新型抗结核药物至关重要的一步。调节子是指病原菌中被同一个转录因子调控的基因或者操纵子的集合，对调节子的全基因组筛选及系统研究是了解病原菌调控机制的基础，然而，尝试遍历所有实验条件来进行调节子的基因组筛选是不现实的。本项目通过计算生物学技术：Bi-cluster双聚类及Pathway能量动态曲线实现结核分枝杆菌细胞壁合成相关调节子的全基因组筛选及网络调控建模，并且采用实验室前期构建的Myco-MarT7转座子突变系统，验证靶基因突变前后病原菌的生物学性状、细胞壁超微结构及组分等变化，实现结核分枝杆菌细胞壁合成相关调节子的功能注释。本项目研究的特点是学科交叉合作与互补，这是已往生物学研究及计算机运算都无法实现的，为其它病原菌的同类研究提供新思路。

The cell wall of mycobacterium tuberculosis plays an important role in maintaining its morphological integrity, resisting the erosion of chemical substances and immune escape. Therefore, high-throughput screening of cell wall synthetic genes is a crucial step in the development of new anti-tb drugs. Regulon refers to the collection of genes or manipulators regulated by the same transcription factor in the pathogenic bacteria, and genome-wide screening and systematic study of the modulation is the basis for understanding the regulatory mechanism of pathogenic bacteria. However, trying to traverse all experimental conditions to make genome screening of regulons is not realistic. This project through computational biology technology: Bi-cluster double clustering and Pathway energy dynamic curve to achieve genome-wide screening and network regulation of tuberculosis related regulator of Mycobacterium tuberculosis, and the use of pre-laboratory construction Myco-MarT7 turn. The mutation of the retrotransposon system to verify the biological characteristics of the pathogenic bacteria before and after the mutation of the target gene, the ultrastructure and composition of the cell wall, and to realize the functional annotation of the related syntheses of the cell wall synthesis of the Mycobacterium tuberculosis. The research of this project is characterized by cross-disciplinary cooperation and complementarity, which can not be achieved by previous biological studies and computer operations, providing new ideas for similar studies on other pathogenic bacteria.