木薯是热带地区的主要粮食作物，然而木薯细菌枯萎病严重危害木薯产量；而木薯应答细菌枯萎病的分子机理还不清楚，相关基因的鉴定非常有限。90kD热激蛋白（HSP90）是进化上高度保守的分子伴侣，在植物的生长发育、胁迫和抗病反应至关重要。申请人克隆了受细菌枯萎病致病菌调节的MeHSP90s，鉴定了其在抗病反应中的作用，同时通过酵母双杂交筛选并结合Pull down和双分子荧光互补验证了MeHSP90的互作蛋白（分子伴侣、自噬蛋白、WRKY和过氧化氢酶）。本项目拟系统鉴定MeHSP90形成的蛋白复合体，同时鉴定MeHSP90上游转录因子；通过基因功能分析、激素互作和信号转导解析蛋白复合体调节的对细菌枯萎病抗病反应机理，构建新的遗传网络和信号途径分支。本研究将全面揭示MeHSP90及互作蛋白（特别是自噬蛋白）在调节抗病反应的功能，为木薯抗病遗传改良提供基因资源和理论依据，从而有效控制木薯细菌枯萎病害。

Cassava is one major tropical crop, however, cassava bacterial blight seriously harm the yield of cassava. To date, the underlying molecular mechanism of cassava in response to bacterial blight is largely unknown, the identification and utilization of disease-related genes are very limited. The 90 kD heat shock protein (HSP90) is one type of evolutionarily conserved molecular chaperones, and it is essential for in plant growth and development, abiotic stress and immune response. In previous studies, we cloned some cassava bacterial blight underlying bacterial pathogen-regulated MeHSP90s, and identified the role of them in defense response to cassava bacterial blight. Moreover, we screen MeHSP90-interacted proteins (including some molecular chaperones and autophagy-related proteins (ATGs), WRKYs and catalase) by yeast two hybrid and confirm the interaction by pull down and bimolecular fluorescence complementation. This project intends to identify MeHSP90-MeATGs formed complex and the transcription factors in the upstream of them. Through the analysis of gene function and signal transduction, we will investigate the MeHSP90-MeATGs formed protein complexes- and transcriptional module-mediated defense response to cassava bacterial blight, and further build new genetic networks and signaling pathway branch. Based on these results, we may improve the disease resistance of cassava, provide the gene resource and theoretical foundation for genetic breeding of disease resistant cassava, and further control cassava disease.