纳米材料的毒性受到越来越多的关注，研究其在细胞的代谢及其造成的小分子代谢物水平变化是研究纳米毒性的有效途径之一。检测生物样本必须借助于样品前处理技术。填充吸附微量萃取法（MEPS）是2004年出现的一种新型固相微萃取形式。其特点为1-2mg 的吸附剂，样品用量小于1ml，解吸溶剂小于100ul，非常适合宝贵的生物样品制备。本项目利用此技术进行金纳米材料干预细胞成长后，得到的培养液和细胞内含物的样品前处理。包括吸附材料的制备、MPES的组装、萃取条件的优化、样品的分析方法建立，并对金纳米干预细胞导致的小分子代谢物含量变化数据进行分析，探讨其在细胞的代谢历程及对典型代谢物代谢水平的影响，阐述其在医药方面潜在应用与毒性。

The toxicity of nano materials is paid more and more attention. Studying on their metabolism and their inducing action to the abnormal metabolism of small molecules in the cells is a valuable way to understand the toxicity. It is necessary to use sample pretreatment for analyzing biological samples. Micro extraction by packed sorbent (MEPS) is a new extraction method, which was introduced firstly by the Swedish named Lars G Blomberg in 2004. The sorbent used in MEPS is only 1-2 mg, the sample consumption is less than 1 ml and the desorption solvent is less than 100 μl. MEPS is very suitable for the precious biological samples. The project would apply the new extraction method to pretreat the samples from cell culture media and cell content resulted from the Au nano materials interference. The works include the preparation of new adsorbent, package of MEPS, optimization of extraction and analysis conditions, the metabolism data analysis and further explanation. The project results would be useful for the application of Au nano materials in medicine.