为了获得新型的实用硫化氢探针，本项目从源头创新，拟利用硫化氢强的还原能力，构建几个基于亚砜为识别功能团的荧光探针：选用BODIPY染料作为发色团。利用分子工程，对含亚砜功能团的BODIPY分子结构进行合理设计，通过引入不同的电子受体单元、改变亚砜功能团的立体位阻作用等，调控亚砜的反应活性，获得与硫化氢可特异反应的BODIPY探针，以获得吸收、发光光谱对硫化氢呈现显著红移的功能分子为最终目标。发展几个两亲聚合物，通过超分子组装手段，与设计合成的小分子组装，制备基于胶束的纳米颗粒，解决有机小分子水溶性差、易光漂白的特点，显著提高与硫化氢的反应活性及响应速度，获得性能优异、实用性的探针体系；发展一种通过核壳结构的纳米颗粒阻止竞争物种与荧光探针接触的设计思路制备新一代硫化氢荧光探针。

To construct novel H2S probes for practical applications in bioimaging, new boron dipyrromethenes (BODIPY) are designed as the versatile platforms. The design strategy relies on reducible property of sulfoxide function, fully employing the reductant feature of H2S. In search of approaches for obtaining an optimal probe for H2S, we explore the possibility of tuning the reactivity of sulfoxide moieties by introducing electron-withdrawing units for appending to BODIPY core and steric substituents for blocking the accesse to competing biothiols, and high selective probes with near-infrared absorption and emission are thus created. Then self-assembling nanoparticles of amphiphilic polymers are employed to encapsulate these new small molecule-based probes into the hydrophobic interior, in this way we establish micellar nanoprobes for fast and selective response to H2S with features of aqueous solubility, excellent photostability and biocompatibility. Importantly, we present a novel approach employing core-shell structured nanoparticles to prevent competing biothiols from accessibility to these sulfoxide sites for engineering new generation of chemoselective probes. We believe that such strategy should indeed be a breakthrough in the construction of sensor systems.