前列腺素类化合物作为局部激素通过激活其特定受体在排卵、受精以及胚胎发育等生殖过程中发挥着重要作用，但目前其对子宫内膜的作用机制尚不清楚。本项目为了阐明PGF2α和PGE2对奶牛子宫内膜的修复与保护作用机制，采用siRNA基因沉默技术、膜片钳技术、Elisa法、荧光定量PCR法、免疫组化染色法等，研究PGF2α和PGE2对奶牛子宫内膜上皮细胞合成分泌VEGF、CTGF、TGF、IL-8、β－防御素、黏液素等细胞组织生长或黏膜保护相关因子的作用；研究PGF2α和PGE2对子宫内膜组织修复与保护作用；研究LPS－PGE2－EP受体途径对前列腺素介导的子宫内膜修复与保护的影响；研究患子宫内膜炎病牛的子宫内膜表达COX、VEGF、CTGF、TGF、IL-8、β－防御素等的变化。项目成果可为临床上合理应用前列腺素类药物或非甾体类抗炎药防治奶牛子宫内膜炎性不孕症提供理论依据和实验基础。

Prostaglandins, as a local hormones, which through activating their specific receptors play an important role in female reproductive processes such as ovulation, fertilization and embryo development. But the physiological effect of prostaglandins in endometrum remain undefined. In this research, in order to clarifying the tissue repair and protective effects of prostaglandin F2α and E2 in bovine endometrium, the contents of these experiments are to investigate the effects of prostaglandin F2α and E2 in bovine endometrial epithelial cells expression and secretion of VEGF, CTGF, TGF, IL-8, mucin and β－defensin that are regarded as factors of tissue repair or protection; to invetigate the role of prostaglandin F2α and E2 on endometrial repair and protection; to identify the influence of the LPS-PGE2-EP receptor pathway in prostaglandin mediated endometrial repair and protection; to examine the alteration of VEGF, CTGF, TGF, IL-8, mucin and β－defensin expression in endometrium which isolated from bovine that diagnosed endometritis, by siRNA transfection, Elisa, real time PCR , patch clamp system and immunohitochemical staining. The results from this research will provide theory evidence and experimental evidence for clinical treatment of endometriun-caused infertility by using prostaglandin preparations or non-steroid anti-inflammatory drugs.