刺激-响应性分子印迹复合微球的组装及其可控吸附与释放性能研究

针对分子印迹聚合物材料对模板分子深层次包埋、模板分子洗脱残留等关键共性问题，本项目从分子结构设计-主客体分子间作用力-印迹分子吸附、脱附行为入手，将刺激-响应性偶氮苯型有机分子与无机材料硅胶通过适当长度的间隔基团连接起来,合成一类对活性分子具有很好的刺激-响应性、高选择性识别以及脱附比较完全的分子印迹复合微球；研究分子印迹层厚度与模板分子包埋的相关性及外界刺激强度-模板分子脱除效率的关联性，从而筛选出最优化的制备功能材料的方法；通过对所合成的刺激-响应性分子印迹复合微球进行吸附结合能力、分子特异性识别能力、刺激-响应性控制目标分子的吸收和释放等物理和化学表征研究，对复杂样品体系中目标分子进行选择性控制吸附与释放,进而对目标分子进行富集及一步纯化。项目的成功,将解决分子印迹技术中模板分子包埋以及洗脱残留等瓶颈问题,进而推进分子印迹技术在固相萃取、色谱分析、传感器、仿生催化等方面的应用。

In this proposal, based on the design of molecular structure, the host-guest intermolecular interaction, and imprinting molecule adsorption and desorption,molecularly imprinted polymeric microsphere was prepared by using stimuli-responsive azobenzene and a range of its derivatives bound to inorganic materials (silica gel) by appropriate isolation spacer, which would be expected to solve the key common problems about molecularly imprinted polymeric materials with template molecule embedding depth and residues. It possesses a stimuli-responsive and selective affinity and desorption with active molecule. The method to prepare functional materials will be optimized by studying the correlation between thickness of surface molecular imprinting and template molecule embedding. Through physical and chemical characterization on the photoresponsive molecularly imprinted polymeric microsphere with adsorption binding capacity, the capability of specific molecular recognition and the stimuli-responsive-regulated release and uptake of target analyte will be investigated.The target molecule in complex sample systems can be selectively adsorped and released, enriched and purified via one-step process. The successful completion of this project will solve the bottleneck problems existing in molecular imprinting technique such as template molecule embedding depth and residues and extend the application of molecular imprinting technique in the progress of solid phase extraction, chromatographic analysis, sensor, and biomimetic catalysis.