One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum
One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum
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一锅法制备硼酸和PEG双功能化二氧化硅颗粒用于大鼠血清中儿茶酚胺的分离纯化
DOI：
10.1039/c5nj01965f
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发表时间：
2015-10
影响因子：
3.3
通讯作者：
Shian Zhong
中科院分区：
文献类型：
--
作者：
Lei Sun;Bingqing Yu;Shaotong Li;Shian Zhong
本文报道了一种简便的一锅法制备氨基苯硼酸（APBA）和聚乙二醇（PEG）双官能化SiO2@APBA@PEG的方法。所制备的纳米粒子不仅可以通过硼酸盐键合选择性地萃取儿茶酚胺，而且可以有效地抵抗PEG刷对蛋白质的吸附; FT-IR光谱和接触角测试证明APBA和PEG基团成功地引入到二氧化硅表面。对多巴胺（DA）和左旋多巴（L-DOPA）的最大吸附容量分别为254.45和104.71 μmol g−1。以该材料为固相萃取剂，从大鼠血清中提取儿茶酚胺。DA和L-DOPA的回收率分别为95 ± 3.21%和93 ± 3.57%。结果表明，SiO2@APBA@PEG颗粒能够有效地从复杂的生物样品中分离纯化儿茶酚胺。
A facile one-pot preparation of aminophenylboronic acid (APBA) and poly(ethyleneglycol) (PEG) bi-functionalized silica particles named SiO2@APBA@PEG by the aid of epoxy silanes is described in this work. The as-prepared particles could not only selectively extract the catecholamine through boronate binding but could also effectively resist protein adsorption using PEG brushes; FT-IR spectra and contact angle measurements proved that APBA and PEG groups were successfully introduced into the surface of silica. The maximum adsorption capacities towards dopamine (DA) and levodopa (L-DOPA) were found to be 254.45 and 104.71 μmol g−1, respectively. The as-prepared material was used as a solid phase extraction (SPE) sorbent for the extraction of catecholamine from rat serum. The recoveries of DA and L-DOPA were 95 ± 3.21% and 93 ± 3.57%, meanwhile, proteins were eliminated. The obtained results indicated that the SiO2@APBA@PEG particles were qualified for efficient purification of catecholamine from complex biological samples.
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影响因子：
6.7
作者：
Shaoxiong Qin;Linlin Wang;Xu Zhang;Gaoshen Su
通讯作者：
Shaoxiong Qin;Linlin Wang;Xu Zhang;Gaoshen Su
影响因子：
6.1
作者：
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影响因子：
3.2
作者：
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影响因子：
3.1
作者：
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影响因子：
14
作者：
Murthy, Ranjini;Shell, Courtney E.;Grunlan, Melissa A.
通讯作者：
Grunlan, Melissa A.