组织间隙液压异常升高是恶性实体瘤的特点并影响肿瘤的放化疗疗效，其测定对肿瘤生物学特性及预后评估具有重要意义，但目前缺乏无创监测肿瘤组织间隙液压的有效手段。课题组前期研究已证实微泡次谐波散射与环境压力之间高度的线性关系以及谐波造影血流灌注评价肿瘤灌注的有效性。那么微泡次谐波散射联合谐波造影血流灌注分析能否有效评价肿瘤组织间隙液压并在其调控治疗中发挥作用，目前尚未见报道。本研究拟建立体内外模型，优化声场参数，通过分析超声RF信号同时计算微泡的次谐波散射幅度和谐波造影血流灌注参数、结合病理评价组织缺氧、血流灌注、间质纤维化、微血管密度、肿瘤增殖活性、转移等，以传统穿刺法测量组织间隙液压为标准，探讨微泡次谐波散射联合谐波造影血流灌注分析在肿瘤组织间隙液压评价及其调控治疗中的作用，为肿瘤生物学特性评估、治疗方案制定、组织间隙液压调控治疗提供有效、无创、价廉的监测手段，对提高肿瘤治疗效果具有重要意义。

Since interstitial fluid pressure (IFP) in most human tumors is highly elevated and will lead to resistance to chemotherapy and radiotherapy, the evaluation of tumor IFP is very important for the assessment of tumor biological characteristics and patient’s prognosis. However, currently there is no effective and noninvasive technique to assess tumor IFP. Our previous studies showed that there is good linear relationship between hydrostatic pressure and subharmonic amplitude and dynamic contrast-enhanced ultrasound could be used to quantify tumor perfusion. However, to the best of our knowledge, there is no article presenting the value of combined subharmonic signal and harmonic perfusion analysis from microbubbles in the evaluation of tumor IFP and its regulation. The purpose of this study is to determine whether combined subharmonic signal and harmonic perfusion analysis from microbubbles enables evaluation of tumor IPF and its regulation, and to compare to the measurements of tumor hypoxia, perfusion, interstitial fibrosis, microvessel density, proliferation activity and metastasis by histopathology, which will provide a noninvasive and cheap imaging technique to the evaluation of tumor biological characteristics, the determination of treatment protocol and the regulation of IFP, and this will improve the cancer treatment.